乳粉能力验证中沙门氏菌的分离鉴定和血清分型
目的 参加编号为NIFDC-PT-220的能力验证, 考核从乳粉中检出沙门氏菌及血清分型能力。方法 参照国标方法对中国食品药品检定研究院提供的随机乳粉样品(CODE140、CODE121)进行沙门氏菌的检验, 并将荧光酶联免疫法和荧光定量PCR技术作为辅助方法。对分离出的疑似菌进行生化鉴定, 并且采用全自动微生物鉴定系统进行全面鉴定。结果 编号CODE140血清分型为O: 4,5,12, H: i; 1,2被确定为鼠伤寒沙门氏菌。编号CODE121未检出。结论 荧光酶联免疫法和荧光定量PCR技术缩短了检测时间, 特异性和敏感性好, 与国标方法配合可确保实验结果的准确性。
Objective To participate in the ability verification of NIFDC-PT-220 to test the ability of detecting Salmonella from milk powder and serotyping. Methods According to the national standard method, the random milk powder samples (CODE140, CODE121) provided by the China Food and Drug Inspection Institute were tested for Salmonella, and the fluorescent enzyme-linked immunoassay and fluorescent quantitative PCR technology were used as auxiliary methods. Biochemical identification of suspected isolated bacteria was performed, and the automatic microbial identification system was used for the overall identification. Results Salmonella typhimurium was detected in CODE 140, the serotype was O: 4,5,12, H: i; 1,2. Salmonella spp. was not detected in CODE 121. Conclusion Fluorescence enzyme - linked immunoassay and fluorescence quantitative PCR shortened the detection time and showed good specificity and sensitivity. Cooperating with the national standard method can ensure the accuracy of the experimental results.
标题：乳粉能力验证中沙门氏菌的分离鉴定和血清分型
英文标题：Proficiency testing of isolation, identification and serotyping of Salmonella in milk powder
作者：
沈锐 重庆市永川食品药品检验所
杨荣荣 重庆市永川食品药品检验所
王梅 重庆市永川食品药品检验所
中文关键词:沙门氏菌,能力验证,分离,鉴定,血清分型,
英文关键词:Salmonella,proficiency testing,isolation,identification,serotyping,
发表日期：2020-03-23
- 文件大小:
- 690.69 KB
- 下载次数:
- 60
-
高速下载
|
|
