马兰头总黄酮的大孔树脂纯化工艺优化
目的 利用大孔树脂来纯化马兰头中粗黄酮, 并确定纯化黄酮的最佳工艺。方法 以黄酮回收率为指标, 在单因素实验的基础上运用Box-Behnken响应面法(response surface methodology, RSM)设计三因素三水平实验以获得最佳纯化条件。结果 HPD-600大孔吸附树脂纯化马兰头粗提液的最佳工艺条件为: 上样浓度0.93 mg/mL、上样pH为3.00、洗脱剂体积分数为84.17%、吸附速率1 BV/h, 洗脱速率1 BV/h, 此条件下马兰头总黄酮的质量分数由纯化前的4.11%提高到纯化后的50.80%。结论 利用HPD-600型大孔树脂可以较好地纯化马兰头中的总黄酮。
Objective To purify crude flavonoids from Kalimeris by macroporous resin and determine the optimum purifying process for flavonoids. Methods Based on the single factor experiment, Box-Behnken response surface methodology, RSM) was used to design a three-factor and three-level experiment to obtain the best purification conditions with flavone recovery rate as the index. Results The optimum conditions for purification of crude extract of Kalimeris with HPD-600 macroporous adsorption resin were as follows: loading concentration 0.93 mg/mL, loading pH 3.00, eluent volume fraction 84.17%, adsorption rate 1 BV/h, and elution rate 1 BV/h. Under these conditions, the mass fraction of total flavonoids in Kalimeris was increased from 4.11% before purification to 50.80% after purification. Conclusion HPD-600 macroporous resin can be used to purify the total flavonoids in Kalimeris.
标题：马兰头总黄酮的大孔树脂纯化工艺优化
英文标题：Optimization of purification process of total flavonoids from Kalimeris by macroporous resin
作者：
沈欣怡 金陵科技学院动物科学与技术学院食品科学系
姚宏亮 金陵科技学院动物科学与技术学院食品科学系
唐艳 金陵科技学院动物科学与技术学院食品科学系
中文关键词:马兰头,总黄酮,大孔树脂,纯化,响应面法,
英文关键词:Kalimeris,total flavonoids,macroporous resin,purification,response surface method,
发表日期：2020-04-01
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