火焰光度检测器-气相色谱法测定食品中3-乙酰基-2,5-二甲基噻吩的含量
目的 建立火焰光度检测器-气相色谱法检测食品中3-乙酰基-2,5-二甲基噻吩(3-acetyl-2,5- dimethylthiophene, ADP)的分析方法。方法 含油脂食品经甲醇提取后冷冻过滤、不含油脂食品经正己烷提取后离心, 以气相色谱仪-火焰光度检测器为检测手段, 外标法定量。结果 ADP在0.005~1.000 μg/mL浓度范围内二次曲线线性良好, 相关系数为0.9999; ADP在不同基质中的检出限均可达到0.015 mg/kg, 定量限达到0.05 mg/kg; 在不同基质及不同加标浓度下, 平均加标回收率为86.3%~108.7%, 相对标准偏差(relative standard deviations, RSDs)为4.50%~6.97%(n=6)。结论 该方法前处理操作简便、技术难度低, 准确度和灵敏度高, 抗干扰能力强, 适用于定量准确并快速地测定食品中ADP的含量。
Objective To establish a method for the determination of 3-acetyl-2,5-dimethylthiophene (ADP) in food by gas chromatography with flame photometric detector. Methods The foods containing fatty were extracted by methanol, frozen and filtered and the foods without fatty were extracted by n-hexane, then centrifuged. Quantitative analysis was carried out by external standard method and the gas chromatograph flame photometric detector was used for detection. Results The linear relationship quadratic curve of ADP in the concentration range of 0.005-1.000 μg/ mL was good, with correlation coefficient 0.9999. The detection limit of ADP in different substrates was 0.015 mg/kg, and the quantitative limit was 0.05 mg/kg. Under different substrates and different concentrations, the average recovery rate was 86.3%-108.7%, and the relative standard deviations (RSDs) was 4.50%-6.97% (n=6). Conclusion This method has the advantages of simple operation, low technical difficulty, high accuracy and sensitivity, strong anti-interference ability, which is suitable for quantitative and accurate determination of ADP in food.
标题：火焰光度检测器-气相色谱法测定食品中3-乙酰基-2,5-二甲基噻吩的含量
英文标题：Determination of 3-acetyl-2,5-dimethylthiophene in food by gas chromatography with flame photometric detector method
作者：
陈克云 山东省食品药品检验研究院
田其燕 山东省食品药品检验研究院
李海霞 山东省食品药品检验研究院
王艳丽 山东省食品药品检验研究院
张卉 山东省食品药品检验研究院
刘艳明 山东省食品药品检验研究院
祝建华 山东省食品药品检验研究院
中文关键词:3-乙酰基-2,5-二甲基噻吩,气相色谱法,火焰光度检测器,
英文关键词:3-acetyl-2,5-dimethyl thiophene,gas chromatography,flame photometric detector,
发表日期：2020-03-20
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