乌饭树果乙醇提取物不同极性分部的体外抗氧化活性研究

乌饭树果乙醇提取物不同极性分部的体外抗氧化活性研究
目的  筛选乌饭树果乙醇提取物中抗氧化活性最强的极性分部。方法  采用石油醚、乙酸乙酯、氯仿、正丁醇和水等5种不同极性溶剂对乌饭树果乙醇提取物进行进一步梯度萃取, 并分析比较其总抗氧化能力(total antioxidant capacity, TAC)、抗脂质过氧化能力及对羟基自由基OH?、超氧阴离子自由基O2-?的清除效果。结果  乌饭树果乙醇提取物的5个不同极性分部均具有一定的抗氧化活性, 但其活性大小差异极显著(P氯仿部>正丁醇部>石油醚部>水部。同时, 在OH?、O2-?自由基清除能力和抗脂质过氧化能力上, 乙酸乙酯部均优于氯仿部和正丁醇部, 且呈明显的量效关系。结论  乌饭树果乙醇提取物的乙酸乙酯部具有最强的抗氧化活性, 且对OH?、O2-?的清除能力及抗脂质过氧化能力均强于L-抗坏血酸及其它部位, 可作为天然抗氧化剂的潜在有效来源。

Objective  To screen the strongest antioxidant activities of polarity fraction of ethanol extract obtained from Vaccinium bracteatum Thunb. fruits. Methods  Totally 5 different polarity solvents (petroleum ether, ethyl acetate, chloroform, n-butanol and water) were used to furtherly fractionate ethanol extracts from Vaccinium bracteatum Thunb. fruits. Moreover, the total antioxidant capacity (TAC), anti-lipid peroxidation and scavenging effect of 5 kinds of polarity fractions on hydroxyl radical (OH?) and superoxide radical (O2-?) were studied and compared. Results  A total of 5 polarity fractions of ethanol extract obtained from Vaccinium bracteatum Thunb. fruits were showed antioxidant activity while exhibited significant differences (P<0.01), among which the ethyl acetate fraction had the strongest TAC, following by the chloroform, n-butanol fractions and the petroleum ether, the water fraction was the weakest. Furthermore, the ethyl acetate fraction had better scavenging capacities of OH?, O2-? and anti-lipid peroxidation than n-butanol fraction and chloroform fraction, and also showed a significant dose-response relationship. Conclusion  The ethyl acetate fraction exhibited the strongest of antioxidant capacity, was superior to L-ascorbic acid and other fractions on the scavenging effects of OH?, O2-? and anti-lipid peroxidation, which could be used as a potential effective source of natural antioxidants.

标题：乌饭树果乙醇提取物不同极性分部的体外抗氧化活性研究
英文标题：Study on the in vitro antioxidant activity of different polarity fractions of ethanol extract obtained from Vaccinium bracteatum Thunb. fruits

作者：
康彬彬 福建生物工程职业技术学院
刘龙燕 福建农林大学食品科学学院
郭溪远 福建农林大学食品科学学院
王祥 福建农林大学食品科学学院
张玲艳 福建农林大学食品科学学院
陈团伟 福建农林大学食品科学学院

中文关键词:乌饭树果,乙醇提取物,极性分部,抗氧化活性,
英文关键词:Vaccinium bracteatum Thunb. fruits,ethanol extract,polar fractions,antioxidant activity,

发表日期：2020-04-13

2025-2-27 20:31 上传 乌饭树果乙醇提取物不同极性分部的体外抗氧化活性研究.pdf

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