液相色谱-串联质谱法测定禽蛋中β-内酰胺类药物残留
目的 建立液相色谱-串联质谱检测禽蛋中药物残留的方法。方法 禽蛋用80%乙腈水溶液提取, PRiME HLB固相萃取小柱净化, 用液相色谱-串联质谱测定, 青霉素G-D7内标法定量。结果 在0.5~20 μg/L的基质匹配标准溶液内, 9种β-内酰胺类药物均呈良好线性关系, 相关系数均大于0.994。样品中9种β-内酰类药物的定量限为1 μg/kg, 在1~10 μg/kg的添加浓度范围内平均回收率为73%~96%, 相对标准偏差小于10%。结论 本方法方便快捷准确, 适于禽蛋中的9种β-内酰胺类药物残留检测。
Objective To establish a method for the detection of drug residues in poultry eggs by liquid chromatography-tandem mass spectrometry. Methods Poultry eggs were extracted with 80% acetonitrile aqueous solution, purified with PRiME HLB solid phase extraction column, determined by liquid chromatography-tandem mass spectrometry, and quantitated by penicillin G-D7 internal standard method. Results In a matrix matching standard solution of 0.5 to 20 μg/L, the 9 kinds of β-lactam showed good linear relationship. The correlation coefficients were all greater than 0.994. The quantitative limit of 9 kinds of β-lactam drugs in the sample was 1 μg/kg, and the average recovery was 73%?96% with the relative standard deviation of less than 10% in the range of 1?10 μg/kg. Conclusion This method is convenient, fast and accurate for the detection of 9 β-lactam residues in poultry eggs
标题：液相色谱-串联质谱法测定禽蛋中β-内酰胺类药物残留
英文标题：Determination of β-lactam in poultry eggs by liquid chromatography -tandem mass spectrometry
作者：
董文婷 湖北省兽药监察所
曾勇 湖北省兽药监察所
吴晓翠 湖北省兽药监察所
周青 湖北省兽药监察所
中文关键词:禽蛋,β-内酰胺类药物,兽药残留,液相色谱-串联质谱法,
英文关键词:poultry eggs,β-lactam,veterinary drug residues,liquid chromatography-tandem mass spectrometry,
发表日期：2020-06-03
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