辽宁省不同来源沙门氏菌耐药性分析
目的 比较辽宁地区食品来源与临床样本来源沙门氏菌的耐药性, 了解不同来源沙门氏菌对14种抗生素的耐药情况, 分析产生差异的原因。方法 用96孔药敏板, 对54份食品来源和55份临床样本来源的沙门氏菌进行14种抗生素的耐药性检测, 通过SPSS统计学软件计算, 同时通过PCR方法对携带耐药基因的整合子目的片段进行扩增, 检测2种来源沙门氏菌Ⅰ类整合子携带情况, 分析其对多重耐药产生的意义。结果 2种来源沙门氏菌对喹诺酮类抗生素—环丙沙星的耐药率有显著差异(食品耐药率高于临床样本: χ2=13.615, P=0.001<0.05); 2种来源沙门氏菌对β-内酰胺类抗生素-头孢噻肟和氨苄西林耐药率有显著差异(临床样本耐药率高于食品样本: 头孢噻肟χ2=4.813, P=0.028<0.05; 氨苄西林χ2=9.653, P=0.002<0.05); 二者多重耐药率有显著差异(临床样本多重耐药率高于食品样本: χ2=4.012, P=0.045<0.05)。二者整合子携带率分别为食品样本22.22%、临床样本29.09%。结论 耐药性的产生与不同环境压力诱导、自身滥用抗生素的习惯有关, 也与Ⅰ类整合子的介导密切相关, 研究结果对环境以及临床抗生素的应用具有指导意义, 以期为进一步探索整合子所携带的耐药基因以及它传递的影响因素提供实验依据。
Objective To compare the drug resistance of Salmonella from food sources and clinical samples in Liaoning province, understand the drug resistance of Salmonella from different sources to 14 kinds of antibiotics, and analyze the causes of drug resistance. Methods The 96-hole drug sensitive plate was used to detect the resistance of Salmonella from 54 food sources and 55 clinical samples for 14 kinds of antibiotic, SPSS statistical software was used to calculate, and at the same time, PCR method was used to amplify the integron fragment carrying drug resistance gene, detect the ClassⅠIntegron carrying status of Salmonella from 2 sources and analyze the significance of ClassⅠIntegron on multi-drug resistance. Results There were significant differences in the resistance of Salmonella from 2 sources to quinolones-ciprofloxacin (drug resistance rate of food samples was higher than clinical sample: ?2=13.615, P=0.001<0.05). Drug resistance of Salmonella isolated from different sources to β-lactam antibiotics-cefotaxime and ampicillin was significantly different (drug resistances rate of clinical samples was higher than food sample: cefotaxime ?2=4.813, P=0.028<0.05, ampicilin ?2=9.653, P=0.002<0.05). There was significant difference in multidrug resistance rate between them (multi-drug resistance in clinical samples was higher than that in food samples: ?2=4.012, P=0.045<0.05). The carrying rate of integron was 22.22% in food samples and 29.09% in clinical samples. Conclusion Drug resistance is related to different external induction, abusing of antibiotics and Class I Integron. The results are significance to application of antibiotics. The results provide experimental basis for further exploration of the drug resistance genes carried by the integrons and the factors affecting their transmission.
标题：辽宁省不同来源沙门氏菌耐药性分析
英文标题：Drug resistance analysis of Salmonella isolated from different sources in Liaoning province
作者：
张铭琰 辽宁省疾病预防控制中心
耿英芝 辽宁省疾病预防控制中心
于淼 辽宁省疾病预防控制中心
张眉眉 辽宁省疾病预防控制中心
中文关键词:沙门氏菌,耐药性,Ⅰ类整合子,临床样本,
英文关键词:Salmonella,drug resistance,Class I Integron,clinical sample,
发表日期：2020-06-11
- 文件大小:
- 379.84 KB
- 下载次数:
- 60
-
高速下载
|
|
