广西火麻膳食纤维提取及其特性研究
目的 优化火麻膳食纤维的提取工艺, 并探索其益生特性。方法 以广西巴马特色食品—火麻为原料, 以火麻膳食纤维提取率为指标, 采用酶-碱法探索料液比、蛋白酶添加量、碱解时间、碱解温度, 碱解pH对火麻膳食纤维提取率的影响, 在此基础上进行响应面优化实验, 再进一步对其有关特性进行研究。结果 在料液比1:10(g:mL), 蛋白酶添加量为2.90%, 碱解时间79 min, 碱解温度58 ℃, 碱解pH=10的条件下, 火麻的总膳食纤维(total dietary fiber, TDF)的提取率达到73.98%。此时, 火麻的可溶性膳食纤维(soluble dietary fiber, SDF)持水力、膨胀率、持油力、二苯代苦味酰基自由基(1,1-diphenyl-2-picrylhydrazyl radical, DPPH)自由基清除率、羟基自由基清除率分别为(2.30±0.22) g/g、(2.26±0.04) mL/g、(4.04±0.20) g/g、(54.29±4.80)%、(34.69±4.65)%。火麻的不溶性膳食纤维(insoluble dietary fiber, IDF)持水力、膨胀率、持油力、DPPH自由基清除率、羟基自由基清除率分别为(1.89±0.01) g/g、(1.17±0.01) mL/g、(4.12±0.24) g/g、(97.25±2.13)%、(96.94±3.75)%。结论 酶-碱法提取的火麻膳食纤维具有良好的持水力及膨胀率, 较高的持油力及抗氧化活性, 并富含多种多糖及单糖物质。
Objective To optimize the extraction process of dietary fiber from flax and explore its probiotic properties. Methods The yield of hemp dietary fiber was used as the experimental index using Guangxi Bama specialty food-hemp(Cannabis sativa L.) as raw material. The effects of material-liquid ratio, protease addition amount, alkaline hydrolysis time, alkaline hydrolysis temperature and alkaline hydrolysis pH on the extraction rate of hemp dietary fiber were explored by using enzyme-alkali method. On this basis, the response surface optimization experiment was carried out, and then the related characteristics were further studied. Results The optimized parameters for the extraction process of hemp dietary fiber were: Material-liquid ratio 1:10 (g: mL), protease addition amount 2.90%, alkaline hydrolysis time 79 min, alkaline hydrolysis temperature 58 ℃, alkaline hydrolysis pH=10. Under these conditions, the extraction rate of total dietary fiber (TDF) from hemp was 73.98%. At this point, soluble dietary fiber of fire linen SDF) free radical scavenging rates and hydroxyl radical scavenging rates were (2.30±0.22) g/g, (2.26±0.04) mL/g, (4.04±0.20) g/g, (54.29±4.80)% and (34.69±4.65)%, respectively, for hydrodynamics, expansion rate, oil-holding capacity, and bitter diphenylhydrazyl radical (1, 1-diphenyldiphenyl2-picrylhydrazyl radical, DPPH). Insoluble dietary fiber (IDF) was (1.89±0.01) g/g, (1.17±0.01) mL/g DPPH free radical clearance (4.12±0.24) g/g, (97.25±2.13)% and (96.94±3.75)%, respectively. Conclusion The hemp dietary fiber extracted by enzyme-alkali method has good water holding capacity and expansion rate, high oil holding capacity and antioxidant activity, and is rich in a variety of polysaccharides and monosaccharides.
标题：广西火麻膳食纤维提取及其特性研究
英文标题：Study on the extraction of Guangxi hemp dietary fiber and its characteristics
作者：
聂梦琳 广西大学轻工与食品学院
饶川艳 广西大学轻工与食品学院
莫明规 广西大学轻工与食品学院
潘海博 广西大学轻工与食品学院
梅丽华 广西大学轻工与食品学院
覃璐琪 广西大学轻工与食品学院
李全阳 广西大学轻工与食品学院
中文关键词:膳食纤维,火麻,响应面法优化,
英文关键词:dietary fiber,hemp,response surface method optimization,
发表日期：2020-05-18
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