北京市朝阳区食物中毒相关金黄色葡萄球菌病原学分析
目的 了解2017年北京市朝阳区11株食物中毒相关金黄色葡萄球耐药性、耐药基因、毒力基因及分子分型。方法 依据GB 4789.10-2016对甲、乙饭店留样食品、餐具及厨师手涂抹样品进行金黄色葡萄球菌分离鉴定; 荧光定量PCR法检测耐药基因mecA、vanA与毒力基因sea、seb、sec、sed、see; 纸片法与微量肉汤稀释法进行药物敏感性试验; 脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)进行溯源分析。结果 甲、乙饭店留样食品、餐具涂抹及厨师手涂抹样品共检出11株金黄色葡萄球菌。耐药基因mecA、vanA与毒力基因sea、seb、sec、sed、see均未检出。检出菌株对青霉素100%耐药, 四环素、氯霉素耐药率分别为27.3%、9.09%, 未发现耐甲氧西林金黄色葡萄球菌株。PFGE分型显示, 甲饭店餐具涂抹菌株、手涂抹菌株及乙饭店所有菌株可为3种带型。结论 甲乙饭店分别为两起由金黄色葡萄球菌引起的食物中毒事件, 皆为携带菌株的厨师污染餐具及食品引起, 菌株具有较高耐药性。
Objective To understand the drug resistance, drug resistant gene, virulent gene and molecular typing of 11 food poisoning-related Staphylococcus aureus in Chaoyang district of Beijing in 2017. Methods The samples of residue food, tablewares and food processing staff’s hands from restaurant A and B were collected for the isolation and identification of Staphylococcus aureus according to GB 4789.10-2016. The drug resistant genes mecA、vanA and virulent genes sea、seb、sec、sed、see were detected by real-time PCR. The drug sensitivity was tested by using K-B assay and broth microdilution according to the clinical and laboratory standard institute (CLSI). Pulse field gel electrophoresis (PFGE) was applied to the source-tracking of this food poisoning accident. Results Eleven strains of Staphylococcus aureus were detected in the samples of residue food, tablewares and food processing staff’s hands in restaurant A and B. The drug resistant genes mecA、vanA and virulent genes sea、seb、sec、sed、see were not detected. The resistant rate to penicillin was 100%, tetracycline and chloramphenicol were 27.3% and 9.09%. Methicillin-resistant Staphylococcus aureus was not found. PFGE typing results revealed that the strains isolated from tableware’s in restaurants A, food processing staff’s hands in restaurants A and all strains isolated from restaurants B were grouped into 3 groups. Conclusion The food poisoning in restaurants A and B were caused by different Staphylococcus aureus which carried by food processing staff contaminated tablewares and food. Those strains showed higher drug resistance.
标题：北京市朝阳区食物中毒相关金黄色葡萄球菌病原学分析
英文标题：Etiological analysis of Staphylococcus aureus associated with food poisoning in Chaoyang district of Beijing
作者：
郝民 北京市朝阳区疾病预防控制中心
王恒伟 北京市朝阳区疾病预防控制中心
邵希凤 北京市朝阳区疾病预防控制中心
田甜 北京市朝阳区疾病预防控制中心
宋衍燕 北京市朝阳区疾病预防控制中心
中文关键词:食物中毒,金黄色葡萄球菌,耐药,毒力基因,脉冲场凝胶电泳,
英文关键词:food poisoning,Staphylococcus aureus,drug resistance,virulent gene,pulse field gel electrophoresis,
发表日期：2020-05-13
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