Objective To find out the correlation between the plate pouring method and the paper disk method to detect the total bacterial count in food, and judge whether there is significant difference between the two methods. Methods The samples were treated according to GB 4789.2-2016 National Food Safety Standard-Microbiological Examination of Food-Determination of Total Bacterial Count. Sample diluent was inoculated with plate pouring method, agar was poured into plate, and sample diluent was inoculated with bacteria counting paper by paper disk method. The above two methods were used to detect the total number of colonies of 30 biscuits and cakes and 2 quality control samples randomly selected from the market, and statistical analysis (t-test) was performed. Results There was no significant difference between the results of plate pouring method and paper disk method (P>0.05), and it was applicable to different colony number ranges (0–100 CFU/g, 100–1000 CFU/g, 1000–10000 CFU/g). The evaluation results of 2 kinds of quality control samples were satisfactory. Conclusion The paper disk method can be used to detect the total number of food colonies quickly, which can shorten the detection process and improve the work efficiency.
标题：平板计数法与纸片法检测食品微生物菌落总数的比较研究
英文标题：Comparison of plate counting method with paper slice method for detecting total bacterial count in food
