超高效液相色谱-串联质谱法测定鸡肉、鸡蛋中氟苯尼考和氟苯尼考胺残留
目的 建立超高效液相色谱-串联质谱准确测定鸡肉、鸡蛋中氟苯尼考和氟苯尼考胺残留的分析方法。方法 鸡肉和鸡蛋样品加入D5-氯霉素内标, 经氨化乙酸乙酯混合溶液超声离心提取, 提取液减压蒸馏浓缩后, C18固相萃取柱净化处理, 采用超高效液相色谱-串联质谱法对氟苯尼考和氟苯尼考胺同时进行检测。 结果 氟苯尼考线性范围为0.2~20 μg/L, 检出限为1.0 μg/kg, 定量限为3.0 μg/kg, 方法回收率为88.0%~108.0%, 相对标准偏差为4.7%~6.4%; 氟苯尼考胺线性范围为0.2~20 μg/L, 检出限为1.0 μg/kg, 定量限为3.0 μg/kg, 方法回收率为76.0%~93.1%, 相对标准偏差为4.1%~7.2%。结论 本方法精确、重现性好, 适用于鸡肉、鸡蛋中氟苯尼考和氟苯尼考胺残留量的测定。
Objective To establish a method for the determination of florfenicol and florfenicol amine residues in chicken and hen eggs by ultra performance liquid chromatography-tandem mass spectrometry. Methods Chicken and hen eggs samples were added to the internal standard of D5-chloramphenicol and extracted by ultrasonic centrifugation with ammoniated ethyl acetate mixed solution. After the extract was concentrated under reduced pressure, purified by the C18 solid-phase extraction column, florfenicol and florfenicol amine residues were simultaneous determined by ultra performance liquid chromatography tandem mass spectrometry. Results The linear range of florfenicol was 0.2–20 μg/L, the detection limit was 1.0 μg/kg, the limit of quantification was 3.0 μg/kg, the method recoveries were 88.0%–108.0%, and the relative standard deviations were 4.7%-6.4%. The linear range of florfenicol amine was 0.2–20 μg/L, the detection limit was 1.0 μg/kg, the limit of quantification was 3.0 μg/kg, the method recoveries were 76.0%–93.1%, and the relative standard deviations were 4.1%–7.2%. Conclusion The method is accurate and reproducible, and is suitable for the determination of florfenicol and florfenicol amine residues in chicken and hen eggs.
标题：超高效液相色谱-串联质谱法测定鸡肉、鸡蛋中氟苯尼考和氟苯尼考胺残留
英文标题：Determination of florfenicol and florfenicol amine residues in chicken and hen eggs by ultra performance liquid chromatography tandem mass spectrometry
作者：
王丽英 河北省疾病预防控制中心
任贝贝 河北省疾病预防控制中心
路杨 河北省疾病预防控制中心
刘梦颖 河北省疾病预防控制中心
刘印平 河北省疾病预防控制中心
冯静 河北省疾病预防控制中心
常凤启 河北省疾病预防控制中心
中文关键词:氟苯尼考,氟苯尼考胺,鸡肉,鸡蛋,超高效液相色谱-串联质谱法,
英文关键词:florfenicol,florfenicol amine,chicken,hen eggs,ultra performance liquid chromatography-tandem mass spectrometry,
发表日期：2020-05-09
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