奶粉中一株阪崎肠杆菌的鉴定及其挥发性产物分析
目的 鉴定奶粉中一株阪崎肠杆菌分离菌株F2-1, 采用顶空气相色谱-质谱联用法(headspace gas chromatography-mass spectrometry, HS-GC-MS)分析分离菌株F2-1在液态培养中产生的挥发性代谢产物。 方法 应用VITEK 2 Compact全自动微生物鉴定系统分析分离菌株的生理生化特征; 利用环介导等温扩增技术(loop-mediated isothermal amplification, LAMP)特异性扩增检测分离菌株的16S rDNA, 通过HS-GC-MS分析分离菌株F2-1代谢的挥发性产物。结果 分离菌株F2-1为革兰氏阴性菌, 生理生化特征与阪崎肠杆菌(Enterobacter sakazakii, ES)的相似性为99%, LAMP检测进一步确认为阪崎肠杆菌, 分离菌株F2-1在营养肉汤中代谢的挥发性产物主要有异戊醛、乙醇、异戊醇、3-羟基-2-丁酮、乙酸、正十五烷、正十六烷、正十七烷、1-癸醇。结论 奶粉中分离菌株F2-1鉴定为阪崎肠杆菌, 该菌产生的挥发性产物为阪崎肠杆菌的快速鉴定提供参考。
Objective To identify an isolated strain of Enterobacter sakazakii F2-1 in milk powder, and analyze the volatile metabolites produced by isolate F2-1 in liquid culture by headspace gas chromatography-mass spectrometry (HS-GC-MS). Methods The physiological and biochemical characteristics were elucidated by using VITEK 2 compact automated microbiology system. The 16S rDNA of isolated strains was amplified by loop-mediated isothermal amplification (LAMP). The volatile compounds (VCs) from F2-1 cultured with liquid-state nutrient broth were analyzed by headspace gas chromatography-mass spectrometry (HS-GC-MS). Results The isolated strain F2-1 was a Gram-negative bacterium, and its physiological and biochemical characteristics were 99% similar to Enterobacter sakazakii (ES). It was confirmed as Enterobacter sakazakii by LAMP test. The volatile products metabolized by strain F2-1 in nutrient broth were isovaleraldehyde, ethanol, isoamyl alcohol, 3-hydroxy-2-butanone, acetic acid, n-pentane, n-cetane, n-heptadecane, 1-decanol. Conclusion Bacterial strain F2-1 isolated from the milk power Is identified as Enterobacter sakazakii. The VCs produced by F2-1 will provide basis for the rapid identification of Enterobacter sakazakii.
标题：奶粉中一株阪崎肠杆菌的鉴定及其挥发性产物分析
英文标题：Identification of a strain of Enterobacter sakazakii from milk powder and analysis of its volatile compounds
作者：
唐静 青岛海关技术中心
赵丽青 青岛海关技术中心
贾俊涛 青岛海关技术中心
马晓玲 上海医药集团青岛国风药业股份有限公司
姜英辉 青岛海关技术中心
马云 青岛海关技术中心
王昌军 青岛海关技术中心
李正义 青岛海关技术中心
中文关键词:阪崎肠杆菌,鉴定,挥发性产物,顶空气相色谱-质谱联用法,
英文关键词:Enterobacter sakazakii,identification,volatile compounds,headspace gas chromatography-mass spectrometry,
发表日期：2019-10-21
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