甲醇萃取法在食用油中酚类抗氧化剂检测中的应用研究
目的 对比国标方法与甲醇萃取法萃取食用油中的酚类抗氧化剂。方法 分别用乙酸乙酯:环己烷(1:1, V:V)萃取凝胶渗透色谱仪(gel permeation chromatograph, GPC)进行样品净化气相色谱检测和甲醇萃取冷冻过滤气相色谱检测。结果 甲醇萃取法在检测叔丁基羟基茴香醚(butyl hydroxyl acids, BHA)、2, 6-二叔丁基-4-甲基苯酚(butylated hydroxytoluene, BHT)、 叔丁基对苯二酚(tertiary butylhydroquinone, TBHQ) 3种抗氧化剂在各自的线性范围内, 线性关系良好, 相关系数r大于0.999, 相对标准偏差(relative standard deviation RSD)为0.91%~1.75%, 回收率为89%~98%。结论 该方法简单、快速、准确、实验成本低, 适用于食用油中酚类抗氧化剂的测定。
Objective To compare the extraction of phenolic antioxidants in edible oil by the national standard method and methanol extraction method. Methods Extracted with ethyl acetate: cyclohexane (1:1, V: V), respectively, using a gel permeation chromatograph (GPC) for sample purification gas chromatography and methanol extraction freeze filtration gas chromatography. Results Methanol extraction method for the detection of butyl hydroxyl acids (BHA), 2, 6-di-tert-butyl-4-methylphenol (BHT), tert-butyl hydroquinone (tertiary butylhydroquinone), TBHQ) The three antioxidants have good linearity in their respective linear ranges, the correlation coefficient was greater than 0.999, the relative standard deviations (RSDs) were 0.91% to 1.75%, and the recovery were 89% to 98%. Conclusion This method is simple, rapid, accurate and low in experimental cost, which is suitable for the determination of phenolic antioxidants in edible oils.
标题：甲醇萃取法在食用油中酚类抗氧化剂检测中的应用研究
英文标题：Study on the application of methanol extraction in the determination of phenolic antioxidants in edible oil
作者：
杨李胜 广东省生物工程研究所(广州甘蔗糖业研究所)
刘志鹏 广东省生物工程研究所(广州甘蔗糖业研究所)
柯华南 广东省生物工程研究所(广州甘蔗糖业研究所)
中文关键词:食用油,抗氧化剂,甲醇萃取法,
英文关键词:edible oil,antioxidant,methanol extraction,
发表日期：2019-06-03
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