返回列表 发布新帖

[生物] 甘草查尔酮B及其衍生物的制备及抗宫颈癌活性

10 0
admin 发表于 2025-2-10 01:00 | 查看全部 阅读模式

甘草查尔酮B及其衍生物的制备及抗宫颈癌活性
目的  提取新疆胀果甘草中的总黄酮, 分离纯化甘草查耳酮B, 合成三甲氧查耳酮B, 对比研究其体外抗宫颈癌活性。方法  采用超声辅助乙醇提取法提取总黄酮; 结合聚酰胺与大孔吸附树脂法纯化总黄酮; 通过薄层法从总黄酮中分离纯化单体查耳酮B(化合物I); 同时, 以取代苯乙酮和取代苯甲醛为原料, Claisen-Schmidt碱催化方法合成三甲氧查尔酮B(化合物II); 以人宫颈癌SiHa和HeLa细胞为体外药理实验模型, 以顺铂为阳性对照, 用噻唑蓝比色法对比测定2个单体化合物对宫颈癌细胞增殖的抑制活性。结果  在浓度为1~100 μg/mL时, 化合物I对SiHa和HeLa细胞作用24、48、72 h时的抑制率为2.99%~75.39%; 而化合物II在此浓度范围内的抑制率为0.76%~86.14%。结论  超声辅助乙醇回流提取法联用聚酰胺和大孔树脂柱层析法是制备含量较高的胀果甘草总黄酮的可行方法; 甘草查尔酮B的甲氧基化修饰可显著提高其抗宫颈癌活性。

Objective  To extract the total flavonoids from Xinjiang Glycyrrhiza inflata, isolate and purify the licorice chalcone B, and synthesize trimethylchalcone B to compare their anti-cervical cancer activity in vitro. Methods  Total flavonoids were extracted by ultrasonic assisted ethanol extraction and were purified by polyamide and macroporous adsorption resin, and monomeric licochalcone B (compound I) was separated and purified from total flavonoids by thin layer method. At the same time, using substituted acetophenone and substituted benzaldehyde as raw materials, trimethoxychalcone B (Compound II) was synthesized by Claisen-Schmidt base catalysis. Human cervical cancer SiHa and HeLa cells were used as in vitro pharmacological experimental models, and  was used as positive control.The inhibitory activity of two compounds were determined by methyl thiazolyl tetrazolium method on the proliferation of cervical cancer cells. Results  At a concentration of 1?100 μg/mL, the inhibitory rates of compound I on SiHa and HeLa cells at 24, 48, and 72 h were 2.99%?75.39%, while the inhibition rates of Ccompound II in this concentration range were 0.76%?86.14%. Conclusion  Ultrasound-assisted ethanol reflux extraction and polyamide/macroporous resin adsorption column chromatography are feasible methods for preparing high content flavonoids from Glycyrrhiza inflata. The methoxylation of Glycyrrhiza chalcone B can significantly improve its anti-cervical cancer activity.

标题:甘草查尔酮B及其衍生物的制备及抗宫颈癌活性
英文标题:Preparation of licochalcone B and its derivatives and their anti-cervical cancer activity

作者:
米热古丽·买买提明 新疆医科大学药学院药物化学有机教研室
木合布力·阿布力孜 新疆医科大学药学院药物化学有机教研室
杨争 新疆医科大学药学院
艾孜提艾力•艾海提 新疆医科大学药学院

中文关键词:胀果甘草,甘草查耳酮B,抗宫颈癌活性,
英文关键词:Glycyrrhiza inflata,licochalcone B,anti-cervical cancer activity,

发表日期:2019-07-24
2025-2-4 21:11 上传
文件大小:
512.86 KB
下载次数:
60
高速下载
【温馨提示】 您好!以下是下载说明,请您仔细阅读:
1、推荐使用360安全浏览器访问本站,选择您所需的PDF文档,点击页面下方“本地下载”按钮。
2、耐心等待两秒钟,系统将自动开始下载,本站文件均为高速下载。
3、下载完成后,请查看您浏览器的下载文件夹,找到对应的PDF文件。
4、使用PDF阅读器打开文档,开始阅读学习。
5、使用过程中遇到问题,请联系QQ客服。

本站提供的所有PDF文档、软件、资料等均为网友上传或网络收集,仅供学习和研究使用,不得用于任何商业用途。
本站尊重知识产权,若本站内容侵犯了您的权益,请及时通知我们,我们将尽快予以删除。
  • 手机访问
    微信扫一扫
  • 联系QQ客服
    QQ扫一扫
2022-2025 新资汇 - 参考资料免费下载网站 最近更新浙ICP备2024084428号
关灯 返回顶部
快速回复 返回顶部 返回列表