基于上转换试纸快速检测一次性纸杯热溶出的双酚A
目的 研制基于上转换颗粒(up-conversion particles, UCNPs)和适配体探针的试纸条, 检测一次性纸杯热溶出的双酚A(bisphenol A, BPA)。方法 制备高发光效率的UCNPs, 对其表面进行氨基和链霉亲和素修饰并连接适配体制备荧光探针, 通过优化加样体积和硝酸纤维素膜(cellulose nitrate membrane, NC)种类成功组装试纸条。结果 未修饰的UCNPs粒径约为73 nm, 表面成功修饰氨基和链霉亲和素之后的粒径约为85 nm, 在测试样品体积为200 μL, 以Sartorius CN 140作为硝酸纤维素膜的最优检测条件下, 试纸检测范围为50~ 800 ng/mL, 最低检出限达12.5 ng/mL。该方法应用于纸杯溶出的BPA检测, 加标回收率在94.53%~103.78%之间, 相对标准偏差小于3.75%, 与国家标准高效液相色谱法的测得结果无显著性差异(P>0.05)。结论 用本实验研制的试纸条检测一次性纸杯热溶出的BPA, 灵敏度高、特异性好、检测时间短, 适用于现场检测。
Objective To develop a test strip based on UCNPs and aptamer probes, and test the BPA dissolved from disposable cups. Methods The UCNPs with high luminescence efficiency were prepared and modified with amino-group and streptavidin on its surface. Then, the fluorescent probes were prepared by connecting the aptamer. The test strip was successfully assembled by optimizing the amount of sample added and the type of cellulose nitrate membrane. Results Diameter of bare UCNPs was about 73 nm, after surface modification with amino-group and streptavidin, the diameter increased to about 85 nm. Under the optimal detection condition of Sartorius CN 140 as the cellulose nitrate membrane, with the sample volume of 200 μL, the detection range of strip for BPA was from 50 ng/mL to 800 ng/mL and the limit of detection was 12.5 ng/mL. The method was applied to detect the BPA dissolved from paper cups. The recoveries were between 94.53% and 103.78%, the RSD was below 3.75% and there was no significant difference (P>0.05) between the results obtained by the HPLC from national standard. Conclusion The test strip developed in this experiment can be used to detect the BPA dissolved from the disposable paper cup, which has high sensitivity, good specificity and short detection time, and is suitable for on-site detection.
标题：基于上转换试纸快速检测一次性纸杯热溶出的双酚A
英文标题：Rapid detection of bisphenol A dissolved from disposable cup based on up-conversion test paper
作者：
李巧凤 军事科学院军事医学研究院环境医学与作业医学研究所,天津市环境与食品安全风险监控技术重点实验室；江南大学食品学院
白家磊 军事科学院军事医学研究院环境医学与作业医学研究所,天津市环境与食品安全风险监控技术重点实验室
彭媛 军事科学院军事医学研究院环境医学与作业医学研究所,天津市环境与食品安全风险监控技术重点实验室
王周平 江南大学食品学院
高志贤 军事科学院军事医学研究院环境医学与作业医学研究所,天津市环境与食品安全风险监控技术重点实验室
中文关键词:上转换颗粒,适配体,双酚A,试纸条,
英文关键词:up-conversion particles,aptamer,bisphenol A,test strip,
发表日期：2019-07-09
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