返回列表 发布新帖

[生物] 基于固相基质分散的高效液相色谱-串联质谱法测定茄科蔬菜及其制品中龙葵素的含量

12 0
admin 发表于 2025-2-12 13:30 | 查看全部 阅读模式

基于固相基质分散的高效液相色谱-串联质谱法测定茄科蔬菜及其制品中龙葵素的含量
目的  建立高效液相色谱-串联质谱法测定茄科蔬菜及其制品中龙葵素α-茄碱和α-卡茄碱含量。 方法  样品采用0.1%甲酸水溶液:乙腈=1:1(V:V)提取, C18固相基质分散净化, 经过色谱柱BEH Amide (2.1 mm ×100 mm, 1.7 μm)分离, 以2 mmol/L乙酸铵的0.1%甲酸水溶液: 2 mmol/L乙酸铵的乙腈溶液为流动相进行梯度洗脱, 柱温40 ℃, 流速0.4 mL/min, 多反应模式监测, 外标法定量。结果  α-茄碱和α-卡茄碱在5~1000 ng/mL内线性关系良好(r2>0.999)。α-茄碱方法检出限和定量限范围分别为0.1~1 mg/kg, 0.3~3 mg/kg; α-卡茄碱检出限和定量限分别为0.03~0.3 mg/kg, 0.1~1 mg/kg; 在低、中、高3水平添加下, α-茄碱回收率为90.7%~108.0%, α-卡茄碱回收率为89.8%~102.7%, 相对标准偏差均小于5.6%(n=6)。结论  本方法快速简便、准确度好、灵敏度高等特点, 适用于大批量茄科蔬菜及其制品中α-茄碱和α-卡茄碱的测定。

Objective  To establish a method for determination of solanine α-solanine and α-chaconine in solanaceous vegetables and products of solanaceous vegetable by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods  The sample was extracted with 0.1% formic acid solution:acetonitrile 1:1(V:V) and purified by matrix solid-phase dispers of C18, which was separated by BEH amidechromatographic column  (2.1 mm×100 mm, 1.7 μm), and analyzed by HPLC-MS/MS with 2 mmol/L ammonium 0.1% formic acid solution and 2 mmol/L ammonium formic acid solution acetonitrile solution as the mobile phase on gradient elution program, the column temperature was 40 ℃ and the flow rate was 0.4 mL/min. It was monitored by multi reaction mode, and quantitatively analyzed by external standard method. Results  The α-solanine and α-chaconine had good linear relationships in the concentration of 5?1000 ng/mL (r2>0.999). The limits of detection and limits of quantitation of α-solanin were 0.1?1 mg/kg and 0.3?3 mg/kg, respectively. The limits of detection and limits of quantitation of α-chaconine were 0.03?0.3 mg/kg, 0.1?1 mg/kg, respectively. Under the addition of low, medium and high levels, the recoveries of α-solanine were 90.7%?108.0%, the recoveries of α-chaconine were 89.8%?102.7%, and the relative standard deviations were less than 5.6% (n=6). Conclusion  This method is rapid and simple, accurate and sensitive, which is suitable for determination of α-solanine and α-chaconine in a large scale solanaceous vegetables and products of solanaceous vegetable.

标题:基于固相基质分散的高效液相色谱-串联质谱法测定茄科蔬菜及其制品中龙葵素的含量
英文标题:Determination of solanine in solanaceous vegetables and products of solanaceous vegetable by high performance liquid chromatography-tandem mass spectrometry based on solid phase matrix dispersion

作者:
唐丽君 江西省食品检验检测研究院
匡佩琳 江西省食品检验检测研究院
李雨露 江西省食品检验检测研究院
张文中 江西省食品检验检测研究院
曾林晖 江西省食品检验检测研究院
朱作为 江西省食品检验检测研究院

中文关键词:茄科蔬菜,龙葵素,固相基质分散,高效液相色谱-串联质谱法,
英文关键词:solanaceous vegetables,solanine,matrix solid-phase disperse,high performance liquid chromatography- tandem mass spectrometry,

发表日期:2019-06-26
2025-2-4 21:09 上传
文件大小:
8.05 MB
下载次数:
60
高速下载
【温馨提示】 您好!以下是下载说明,请您仔细阅读:
1、推荐使用360安全浏览器访问本站,选择您所需的PDF文档,点击页面下方“本地下载”按钮。
2、耐心等待两秒钟,系统将自动开始下载,本站文件均为高速下载。
3、下载完成后,请查看您浏览器的下载文件夹,找到对应的PDF文件。
4、使用PDF阅读器打开文档,开始阅读学习。
5、使用过程中遇到问题,请联系QQ客服。

本站提供的所有PDF文档、软件、资料等均为网友上传或网络收集,仅供学习和研究使用,不得用于任何商业用途。
本站尊重知识产权,若本站内容侵犯了您的权益,请及时通知我们,我们将尽快予以删除。
  • 手机访问
    微信扫一扫
  • 联系QQ客服
    QQ扫一扫
2022-2025 新资汇 - 参考资料免费下载网站 最近更新浙ICP备2024084428号
关灯 返回顶部
快速回复 返回顶部 返回列表