飞行时间质谱即用型金黄色葡萄球菌质控样品研究
目的 评估即用型金黄色葡萄球菌质控样品在飞行时间质谱技术中的应用价值。方法 将金黄色葡萄球菌质控样品分别在37 ℃和室温环境25 ℃进行运输稳定性实验, 在4及-20 ℃条件下进行储藏稳定性实验, 并评价其稳定性。对保存在-20 ℃条件下不同时间的金黄色葡萄球菌质控样品进行质谱鉴定, 并做VITEK全自动微生物分析系统鉴定及血浆凝固酶试验鉴定。结果 即用型金黄色葡萄球菌质控样品稳定, 质谱图相近, 所得蛋白峰信息仅存微小差异, 匹配度良好。VITEK全自动微生物分析系统及血浆凝固酶实验结果均为金黄色葡萄球菌, 满足飞行时间质谱技术所需质控样品条件。结论 基质辅助激光解析电离飞行时间质谱技术直接检测金黄色葡萄球菌具有简单快速、准确性高等优点, 相较传统方法操作简单, 检验结果更加直观, 为飞行时间质谱技术鉴定金黄色葡萄球菌提供及时、准确的依据。
Objective To evaluate the application value of ready-to-use Staphylococcus aureus (S. aureus) quality control samples in time-of-flight mass spectrometry. Methods The stability of S. aureus quality control samples was tested at 37 ℃ and room temperature 25 ℃ respectively, and the storage stability was test at 4and ?20 ℃, and the stability was evaluated. The quality control samples of Staphylococcus aureus stored at ?20 ℃ for different periods were identified by mass spectrometry, VITEK automatic microbial analysis system and plasma coagulase test. Results The ready-to-use Staphylococcus aureus quality control samples were stable and the mass spectra were similar. The protein peak information obtained has only slight differences and good matching. The results of the VITEK automatic microbiological analysis system and the plasma coagulase experiment were both Staphylococcus aureus, which meet the quality control sample conditions required by time-of-flight mass spectrometry. Conclusion MALDI-TOF MS technology for direct detection of Staphylococcus aureus has the advantages of simple, rapid and high accuracy. Compared with traditional methods, the operation is simple and the test results are more intuitive, so as to provide a timely and accurate basis for the identification of Staphylococcus aureus by time-of-flight mass spectrometry.
标题：飞行时间质谱即用型金黄色葡萄球菌质控样品研究
英文标题：Study on quality control samples of ready-to-use Staphylococcus aureus by time-of-flight mass spectrometry
作者：
张涛 河北省食品检验研究院,河北省食品安全重点实验室
张雅伦 河北省食品检验研究院,河北省食品安全重点实验室
刘广鹏 河北省食品检验研究院,河北省食品安全重点实验室
陈晨 河北省食品检验研究院,河北省食品安全重点实验室
郭楠楠 河北省食品检验研究院,河北省食品安全重点实验室
李献 河北省食品检验研究院,河北省食品安全重点实验室
周巍 河北省食品检验研究院,河北省食品安全重点实验室；河北师范大学生命科学学院
中文关键词:金黄色葡萄球菌,质控样品,基质辅助激光解析电离飞行时间质谱,
英文关键词:Staphylococcus aureus,standard sample,matrix-assisted laser analytical ionization time-of-flight mass spectrometry,
发表日期：2020-01-03
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