云南乳饼中金黄色葡萄球菌污染状况及其耐药性分析
目的 了解云南省乳饼中主要致病菌的污染状况及耐药情况。方法 采集云南省乳饼主产地区样品62件, 用传统方法结合基质辅助激光解吸电离飞行时间质谱进行分离鉴定, 采用微量肉汤稀释法对金黄色葡萄球菌进行12种抗生素敏感性分析。结果 共检出18株金黄色葡萄球菌, 检出率为29.03%, 沙门氏菌和单增李斯特氏菌均未检出。农贸市场、加工店和奶牛养殖户检出率分别为44.44%、26.67%和16.67%(P=0.321), 超市和网店的样品均未检出; 散装和包装样品检出率分别为34.38%、23.33%(P=0.433)。所有菌株的耐药性如下: 青霉100%, 红霉素44.44%, 复方新诺明33.33%, 苯唑西林、克林霉素和头孢西丁的耐药率均为27.78%, 四环素22.22%, 多重耐药率为72.22%。18株菌对环丙沙星、达托霉素、万古霉素、氯霉素和庆大霉素均为敏感。结论 金黄色葡萄球菌为乳饼中的主要食源性致病菌, 其对抗生素耐药性较普遍, 需加强畜禽养殖及乳饼加工卫生条件监管。
Objective To understand the contamination and drug resistance of Staphylococcus aureus in milk cake in Yunnan province. Methods A total of 62 samples from the main regions of Yunnan milk cake were collected, then separated and identified by traditional methods combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and the sensitivity analysis of 12 antibiotics against Staphylococcus aureus was carried out by micro-broth dilution. Results A total of 18 strains of Staphylococcus aureus were detected, with a detection rate of 29.03%. Salmonella and Listeria monocytogenes were not detected. The detection rates of farmers' markets, processing shops and dairy farming households were 44.44%, 26.67% and 16.67%, respectively (P = 0.321). The samples of supermarkets and online stores were not detected. The detection rates of bulk and packaging samples were 34.38%, 23.33%, respectively (P = 0.433). The drug resistance of all strains was as follows: Penicillium 100%, erythromycin 44.44%, compound sulfamethoxazole 33.33%, the resistance rates of oxacillin, clindamycin and cefoxitin were 27.78%, tetracycline was 22.22%, and the multiple drug resistance rate was 72.22%. The 18 strains were sensitive to ciprofloxacin, datomycin, vancomycin, chloramphenicol and gentamicin. Conclusion Staphylococcus aureus is the main food-borne pathogen in milk cakes, and its resistance to antibiotics is more common. It is necessary to strengthen the supervision of livestock and poultry breeding and health conditions of milk cake processing.
标题：云南乳饼中金黄色葡萄球菌污染状况及其耐药性分析
英文标题：Analysis on contamination status and drug resistance of Staphylococcus aureus in Yunnan milk cake
作者：
朱静静 云南农业大学食品科学技术学院
杨嘉颖 云南农业大学食品科学技术学院
杨越超 云南农业大学食品科学技术学院
邹颜秋硕 云南省疾病预防控制中心
任翔 云南省疾病预防控制中心
汤晓召 云南省疾病预防控制中心
高斌 云南农业大学食品科学技术学院
黄艾祥 云南农业大学食品科学技术学院
中文关键词:乳饼,金黄色葡萄球菌,分离,鉴定,耐药性,
英文关键词:milk cake,Staphylococcus aureus,isolation,identification,drug resistance,
发表日期：2020-03-16
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