QuEChERS-高效液相色谱-串联质谱法测定鸡蛋中38种药物残留
目的 建立高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry, HPLC-MS/MS)测定鸡蛋中38种药物残留的分析方法。方法 样品采用0.1 mol/L乙二胺四乙酸二钠(ethylene-diamine-tetra-acetate, EDTA)和乙腈提取, 经QuEChERS净化后, 通过LC-MS/MS测定, 外标法定量。结果 鸡蛋中38种药物在0.3~100 μg/kg浓度范围内相关系数(r2)在0.99138~0.99989之间, 线性关系良好。方法检出限0.05~1.0 μg/kg, 定量限0.1~3.0 μg/kg, 在3.0、10.0、50.0 μg/kg 3个添加水平的平均回收率72.49%~114.06%, 相对标准偏差(n=6)2.37%~13.85%。结论 本方法操作简便快速、灵敏稳定, 适用于鸡蛋中多种药物残留的高通量快速检测。
Objective To establish a method for determination of 38 kinds of veterinary drugs in eggs by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The sample was extracted with 0.1 mol/L EDTA and acetonitrile, and cleaned up by QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction method. Then determination of the analytes was performed by LC-MS/MS in negative and positive ionization mode using an electrospray ionization source adopting the multi-reaction monitoring mode. Quantitative was achieved by an external standard method. Results The correlation coefficient (r2) of 38 kinds of drugs in eggs was 0.99138-0.99989 in the concentration range of 0.3?100 μg/kg, and the linear relationship was good. Methods the detection limit was 0.05?1.0 μg/kg, the quantitative limit was 0.1?3.0 μg/kg. The average recoveries of the 38 veterinary drugs were ranged from 72.49% to 114.06% at 3 spiked levels of 3.0, 10.0 and 50.0 μg/kg, with the relative standard deviations (RSDs) in the range of 2.37%-13.85%. Conclusion The proposed method is simple, fast, sensitive and accurate, which is suitable for fast high-throughput detection of multiple residues in eggs.
标题：QuEChERS-高效液相色谱-串联质谱法测定鸡蛋中38种药物残留
英文标题：Determination of 38 kinds of veterinary drugs in eggs by QuEChERS and high performance liquid chromatography tandem mass spectrometry
作者：
王娇 海南省动物疫病预防控制中心
李庆霞 海南省动物疫病预防控制中心
韵小娟 海南省动物疫病预防控制中心
谢玉荣 海南省动物疫病预防控制中心
杨信 海南省动物疫病预防控制中心
中文关键词:鸡蛋,多残留检测,液相色谱-串联质谱法,QuEChERS,
英文关键词:eggs,multi-residues,liquid chromatography-tandem mass spectrometry,QuEChERS,
发表日期：2020-03-07
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