Objective To evaluate the uncertainty of determination of sucralose in beverage by high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD). Methods The content of sucralose in beverage was quantitatively analyzed based on GB 22255-2014 Determination of sucralose in food. The uncertainty of the factors in measurement process was evaluated and the synthetic uncertainty and extension uncertainty were calculated according to JJF 1059-2012 Evaluation and expression of uncertainty in measurement. Results The result of HPLC-ELSD in the determination of sucralose in beverages could be expressed as (45.0±4.09) mg/kg, k=2 (P=95%). Conclusion The uncertainty in the determination of sucralose content in beverages mainly comes from the fitting of standard curve and the preparation of standards. In the actual measurement process, the standardized operation is required to reduce the uncertainty factors and improve the accuracy of the measurement results.
标题：高效液相色谱-蒸发光散射检测法测量饮料中三氯蔗糖含量的不确定度评估
英文标题：Uncertainty evaluation for determination of sucralose in beverage by high performance liquid chromatography-evaporative light-scattering detector
