3种不同固定相对皂苷异构体分离度的对比研究

3种不同固定相对皂苷异构体分离度的对比研究
目的  分析3种不同固定相对皂苷异构体的分离效果。方法  3对皂苷异构体gypenoside L和gypenoside LI、damulin A和damulin B、20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3, 通过C18色谱柱、五氟苯基丙基色谱柱及双苯基色谱柱进行分离比较。以Inertsill ODS-SP色谱柱、Shim-pack Velox PFPP色谱柱和Shim-pack Velox Biphenyl色谱柱作为固定相, 用不同比例的流动相分离gypenoside L、gypenoside LI、damulin A、damulin B、20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3。结果  Shim-pack Velox PFPP色谱柱分别在29%和33%乙腈水溶液以1.0 mL/min等度洗脱, gypenoside L和gypenoside LI的分离度达到2.27, 20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3的分离度达到3.50, 分离效果最佳; Shim-pack Velox Biphenyl色谱柱在33%乙腈水溶液以0.5 mL/min等度洗脱, damulin A和damulin B的分离效果最佳, 分离度达到2.73。结论  五氟苯基丙基色谱柱因键合五氟苯基丙基, 对差向异构体具有更好的分离度, 双苯基色谱柱因键合二苯基, 对位置异构体有更好的分离度。

Objective  To analyze the separation effects of 3 different stationary phases on saponin isomer. Methods  Three pairs of saponin isomers, gypenoside L and gypenoside LI, damulin A and damulin B, 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3, were compared by C18 column, pentafluorophenylpropyl (PFPP) column and diphenyl column, respectively. Inertsill ODS-SP column, Shim-pack Velox PFPP column and Shim-pack Velox Diphenyl column were used as stationary phases, and gypenoside L, gypenoside LI, damulin A, damulin B, 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 were separated by different proportions of mobile phase. Results  The effects of separation of gypenoside L and gypenoside LI, and 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 showed the best using Shim-pack Velox PFPP column. The resolution of gypenoside L and gypenoside LI was 2.27 eluted with 29% acetonitrile and the resolution of 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 was 3.50 eluted with 33% acetonitrile at flow rate of 1.0 mL/min; Damulin A and damulin B showed the best separation, with the resolution of 2.73, using Shim-pack Velox Diphenyl column eluted with 33% acetonitrile at the flow rate of      0.5 mL/min. Conclusion  Pentafluorophenylpropyl column is used to separate the epimers due to the bonding of pentafluorophenylpropyl. Diphenyl column shows better resolution for positional isomers due to the bonding of diphenyl groups.

标题：3种不同固定相对皂苷异构体分离度的对比研究
英文标题：Comparative studies on resolution of saponin isomers by various stationary phases

作者：
李心璨 中央民族大学药学院
唐天昕 中央民族大学药学院
余辰茜 中央民族大学药学院
杨飞雪 中央民族大学药学院
朴香兰 中央民族大学药学院

中文关键词:皂苷,同分异构体,分离,五氟苯基丙基色谱柱,双苯基色谱柱,
英文关键词:saponin,isomer,separation,pentafluorophenylpropyl column,biphenyl column,

发表日期：2019-12-20

2025-2-4 21:01 上传 3种不同固定相对皂苷异构体分离度的对比研究.pdf

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