Objective To confirm of the uncommonly suspected Salmonella isolated from the unknown sample. Methods Isolation and purification of suspected Salmonella were conducted according to the GB 4789.4-2016 National food safety standard-Food microbiological examination-Salmonella, and it was confirmed by biochemical identification, 16S sequencing, Ribo-printer automatic microbial gene fingerprint identification system, MALDI Biotyper microbial identification time of flight mass spectrometry and serotyping. Results The identification result of automatic bacterial identification/drug sensitivity system was Escherichia coli. The identification result of 16S sequencing was Salmonella. The identification result of gene fingerprint identification system was Salmonella arizonae/IIIb. The identification result of was Salmonella enterica subsp. diarizonae, and serotyping also indicated that the type was IIIb, so the detected bacteria should be Salmonella enterica subsp. diarizonae. Conclusion The combination of multiple identification methods can ensure the reliability of the experimental results.
标题:1株罕见的可疑沙门氏菌鉴定结果的确认与分析
英文标题:Identification and analysis of the uncommonly suspected Salmonella