QuEChERS技术结合高效液相色谱-串联质谱法快速测定动物源性食品中克伦特罗的残留量
目的 建立一种高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry, HPLC-MS/MS)快速检测动物源性食品中克伦特罗残留量。方法 样品经β-葡萄糖醛甘酶酶解, 乙腈提取, 经过ProElut QuEChERS净化管净化。采用0.1%甲酸(A)和乙腈(B)作为流动相进行梯度洗脱, 用多反应监测模式(multiple reaction monitoring, MRM)对克伦特罗含量进行检测。结果 该方法可以在15 min内完成目标物的分离分析。克伦特罗在0.5、1、5 μg/kg添加水平的回收率为90.0%~94.3%, 相对标准偏差小于10%(n=6), 方法检出限为0.5 μg/kg。结论 该方法快速、准确、灵敏, 适合用于动物源性食品中克伦特罗的残留量的测定。
Objective To establish a method for determination of clenbuterol residues in animal-derived foods by QuEChERS technique with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The sample was digested with β-glucuronide, extracted with acetonitrile, and purified by a ProElut QuEChERS purification tube. Gradient elution was carried out using 0.1% formic acid (A) and acetonitrile (B) as mobile phases, and clenbuterol content was detected by multiple reaction monitoring (MRM). Results The sample could be separated and analyzed within 15 min. The recoveries of clenbuterol at 0.5, 1, 5 μg/kg were 90.0%-94.3%, and the relative standard deviations were less than 10% (n=6). The detection limit of the method was 0.5 μg/kg. Conclusion The method is rapid, accurate and sensitive, which is suitable for the determination of clenbuterol residues in animal-derived foods.
标题：QuEChERS技术结合高效液相色谱-串联质谱法快速测定动物源性食品中克伦特罗的残留量
英文标题：Rapid determination of clenbuterol residues in animal-derived foods by QuEChERS technique with high performance liquid chromatography-tandem mass spectrometry
作者：
张鑫 吉林省食品检验所;吉林省安信食品技术服务有限公司
刘文竹 吉林省食品检验所;吉林省安信食品技术服务有限公司
康优 吉林省农业科学院
王月娇 吉林省药品检验所
李滢倩 吉林省食品检验所
石金娥 吉林省食品检验所
刘斌 吉林省食品检验所
中文关键词:克伦特罗,高效液相色谱-串联质谱法,ProElut QuEChERS,
英文关键词:clenbuterol,high performance liquid chromatography-tandem mass spectrometry,ProElut QuEChERS,
发表日期：2019-02-01
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