几种不同血清型沙门氏菌生化结果及基质辅助激光解吸电离飞行时间质谱离子峰的差异性分析

几种不同血清型沙门氏菌生化结果及基质辅助激光解吸电离飞行时间质谱离子峰的差异性分析
目的  了解不同血清型沙门氏菌生化结果差异及飞行质谱离子峰的差异性。方法  选取10种不同血清型的沙门氏菌, 确认其血清种类, 使用VITEK2全自动微生物鉴定仪进行生化分析, 采用基质辅助激光解吸电离飞行时间质谱法(matrix-assisted laser desorption ionization time-of-flight mass spectrometry, MALDI-TOF- MS)获得蛋白质指纹图谱, 并进行离子峰差异性分析。结果  10株不同血清型沙门氏菌尿素酶、赖氨酸、H2S等33种生化结果无差异, 而L-脯氨酸芳胺酶、乳酸盐产碱、O/129耐受等14个反应结果有个别差异; 建立了10株沙门氏菌鉴定分型主要差异的离子峰数据库, 并发现菌株存在完全不同的离子峰: Sal-01具有8370.144离子峰, Sal-03具有5462.553、10927.51离子峰, Sal-06具有3013.925、6035.049离子峰, Sal-07具有3030.747、6026.629、6067.12离子峰。结论  不同血清型沙门氏菌在生化结果上有一定的差异, 通过MALDI-TOF-MS分析能够获得不同血清型的沙门氏菌具有差异性离子峰基础数据。

Objective  To explore the differences of biochemical results and ionic peaks in time-of-flight mass spectrum from different serotypes of Salmonella. Methods  A total of 10 different serotypes of Salmonella were selected and the serum types were confirmed. The VITEK2 automatic microbial identification instrument was used for biochemical analysis, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to obtain protein fingerprints and analyze the difference of ion peaks. Results  There were no differences in 33 biochemical results of urease, lysine, H2S and so on in 10 serotypes of Salmonella, while there were individual differences in 14 reaction results of L-proline aramine enzyme, alkali production of lactate, O/129 tolerance and so on. A total of 10 ion peak databases with different identification types of Salmonella were established, and it was found that the strains had completely different ion peaks: Sal-01 had 83701.14 ion peak, Sal-03 had 5462.553, 10927.51 ion peaks, Sal-06 had 3013.925 and 6035.049 ion peaks, and Sal-07 had 3030.747, 6026.629, 6067.12 ion peaks, respectively. Conclusion  There are some differences in biochemical results between different serotypes of Salmonella. Basic data of different ionic peaks of Salmonella with different serotypes can be obtained by MALDI-TOF-MS analysis.

标题：几种不同血清型沙门氏菌生化结果及基质辅助激光解吸电离飞行时间质谱离子峰的差异性分析
英文标题：Biochemical results of several different serotypes of Salmonella and divergence analysis of matrix-assisted laser desorption ionization time-of-flight mass spectrometry ion peaks

作者：
宋安华 广州市食品检验所
肖剑 广州市食品检验所
陈楷 广州市食品检验所

中文关键词:沙门氏菌,血清型,生化鉴定,基质辅助激光解吸电离飞行时间质谱,
英文关键词:Salmonella,serotype,biochemical identification,matrix-assisted laser desorption ionization time-of-flight mass spectrometry,

发表日期：2019-07-01

2025-2-2 20:08 上传 几种不同血清型沙门氏菌生化结果及基质辅助激光解吸电离飞行时间质谱离子峰的差异性分析.pdf

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