不同品种禽蛋中胆碱含量测定与分析研究
目的 建立酶比色法测定禽蛋中胆碱含量的分析方法。方法 在GB 5413.20-2013《食品安全国家标准 婴幼儿食品和乳品中胆碱的测定》方法的基础上对检验条件(称样量、水解时间)进行优化。并比较分析不同品种禽蛋全蛋和蛋黄中胆碱含量水平和差异。结果 称样量和水解时间优化结果为: 全蛋和蛋黄称样量分别为1.0~2.0 g、0.5~1.0 g, 水解时间为4 h。胆碱氢氧化物在50~250 μg/mL浓度范围内线性良好(r=1), 样品精密度相对标准偏差2.17%; 样品加标回收率为94.0%~101.2%, 相对标准偏差为1.86%~5.13%。蛋黄与全蛋中胆碱含量呈显著正相关(r=0.499, P＜0.01)。鸡蛋、鸭蛋、鹅蛋、鹌鹑蛋中胆碱含量(蛋黄)有显著差异(P＜0.05), 鹅蛋、鹌鹑蛋中含量高, 其次为鸭蛋、鸡蛋, 其蛋黄中胆碱含量均值分别为1222.5、1277.9、1345.1、 1340.8 mg/100 g。土鸡蛋、乌鸡蛋中胆碱含量(蛋黄、全蛋)显著高于普通鸡蛋(P＜0.05)。乌鸡蛋与土鸡蛋蛋黄、全蛋胆碱含量均无显著差异(P＞0.05)。咸鸭蛋中胆碱含量明显高于生鸭蛋, 蛋黄中含量均值分别为1181.8、 1406.0 mg/100 g, 且差异具有统计学意义(P＜0.001)。结论 本研究建立了禽蛋类胆碱提取测定方法-酶比色法, 并探讨了不同品种禽蛋营养价值, 为消费者合理选择不同品种禽蛋提供参考。
Objective To establish a method for the determination of choline in poultry eggs by enzyme colorimetric method. Methods On the basis of GB 5413.20-2013 Determination of choline in food safety national standard infant food and dairy products, the test conditions (sample size and hydrolysis time) were optimized. and the contents and differences of choline in whole eggs and egg yolks of different varieties of poultry were compared and analyzed. Results The optimized results of symmetrical sample size and hydrolysis time were expressed as follows: the weight sample sizes of whole egg and yolk were 1.0~2.0 g and 0.5~1.0 g respectively, and the hydrolysis time was 4 h. Choline hydroxide had good linear relationships in the range of 50?250 μg/mL (r=1). The relative standard deviation of precision of sample was 2.17%. The standard recovery rates were 94.0%?101.2%, and the relative standard deviations were 1.86%?5.13%. The contents of choline in egg yolk and whole egg were positively correlated (r=0.499, P0.05). The content of choline in salted duck eggs was significantly higher than that in raw duck eggs, and the mean contents of choline in yolk were 1181.8 and 1406.0 mg/100 g respectively, with statistically significant differences (P<0.001). Conclusion This study establishes an enzyme colorimetric method of extraction and determination of choline in poultry eggs, and discusses the nutritional value of different varieties of poultry eggs, so as to provide references for consumers to rationally select different varieties of poultry eggs.
标题：不同品种禽蛋中胆碱含量测定与分析研究
英文标题：Determination and analysis of choline in different varieties of eggs
作者：
冯炜 山东省食品药品检验研究院
于艳艳 山东省食品药品检验研究院
田洪芸 山东省食品药品检验研究院
程月红 山东省食品药品检验研究院
张廷文 山东省食品药品检验研究院
厉玉婷 山东省食品药品检验研究院
李静 山东省食品药品检验研究院
任雪梅 山东省食品药品检验研究院
鲍连艳 山东省食品药品检验研究院
中文关键词:鸡蛋,鸭蛋,鹅蛋,鹌鹑蛋,酶比色法,胆碱,t检验,
英文关键词:eggs,duck eggs,goose eggs,quail eggs,enzyme colorimetry,choline,t test,
发表日期：2019-04-15
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