QD-Infinity免疫荧光定量法快速测定玉米中呕吐毒素的适用性研究
目的 建立QD-Infinity免疫荧光法快速测定玉米中呕吐毒素含量的方法, 为玉米中呕吐毒素现场筛查提供快速、有效的检测/监测手段。方法 通过测定玉米质控样、阴性玉米的添加回收率, 与高效液相色谱(high performance liquid chromatography, HPLC)法测定天然玉米进行比较, 对QD-Infinity免疫荧光测定法的准确度、精密度及其检出限与定量限进行全面评估。结果 玉米质控样中呕吐毒素含量测定值均在其理论值范围内; 回收率结果为109.77%、107.83%和111.01%, 变异系数分别为9.77%、7.83%、11.01%; 与HPLC法相比, 测定26份玉米结果的相对偏差在1.20%~5.78%之间; 该方法的检出限与定量限分别为10 μg/kg、32 μg/kg。结论 QD-Infinity免疫荧光法是一种有效、实用的分析方法, 能用于玉米中呕吐毒素的定量检测。
Objective To establish a method for the determination of vomitoxin in corn by QD-Infinity immunofluorescence, and provide a rapid and effective means of detecting and monitoring vomitoxin in corn. Method The accuracy, precision, limit of detection and limit of quantitation of QD-infinity immunofluorescence assay were evaluated comprehensively by determining the vomitoxin in corn quality control material, detecting addition recovery of negative maize, and comparing the results of detection of natural corn samples by high performance liquid chromatography. Results The content of vomitoxin in corn quality control material (different levels) were in their own scope of accreditation. The recovery rate in negative corn samples were 109.77%, 107.83% and 111.01%, and the variable coefficient were 9.77%, 7.83% and 11.01%, respectively. Compared with HPLC, the relative deviations of results of testing natural corn samples were between 1.20%?5.78%. The limit of detection ju(LOD) was 10 μg/kg, and the limit of quantitation (LOQ) was 32 μg/kg. Conclusion QD-Infinity immunofluorescence assay is an effective and practical method for analysis, which can be used in quantitative detection of vomitoxin in corn.
标题：QD-Infinity免疫荧光定量法快速测定玉米中呕吐毒素的适用性研究
英文标题：Applicability of QD-Infinity immunofluorescence quantitative assay for rapid detection of vomitoxin in corn
作者：
马志 深圳市赛泰诺生物技术有限公司
刘松雁 石家庄畜产品质量监测中心
董明杰 深圳市赛泰诺生物技术有限公司
田梅 石家庄畜产品质量监测中心
赵义良 石家庄畜产品质量监测中心
中文关键词:QD-Infinity免疫荧光测定法,食品饲料玉米,呕吐毒素,定量检测,
英文关键词:QD-Infinity immunofluorescence assay,food corn or feed corn,vomitoxin,quantitative detection,
发表日期：2019-04-15
- 文件大小:
- 356.49 KB
- 下载次数:
- 60
-
高速下载
|
|
