从蔬菜中回收细菌的前处理方法研究
目的 结合细菌的回收效果, 探究生菜和黄瓜微生物检测的最佳样品前处理方法。方法 以生菜和黄瓜为研究对象, 通过电镜观察细菌在经过样品处理后蔬菜中的附着与内化, 结合细菌回收实验设计3种前处理方法, 分别是不同取样量, 不同取样部位和不同稀释方法, 采用SPSS软件对3种处理下细菌的回收结果进行差异性分析。结果 实验设计的3种方法对细菌的回收效果差异显著(P<0.05)。黄瓜和生菜中微生物检测样品前处理方法选取50 g取样量, 并采用匀浆的方式最有效地回收细菌, 取样部位如果考虑将食用风险最大化, 建议选择表面取样, 如果需考虑整体污染水平, 建议对样品表面及内部进行整体取样。结论 改进后的新鲜果蔬前处理方法能更有效地回收到果蔬样品中的细菌, 对制订相关检测标准或是开展果蔬微生物研究样品前处理方法提供了有效建议。
Objective To explore the best sample preparation method for microbial detection of lettuce and cucumber combined with the recovery effect of bacteria. Metheds Taking lettuce and cucumber as research objects, the adhesion and internalization of bacteria in the vegetables after sample treatment were observed by electron microscope. A total of 3 kinds of pretreatment methods were designed in combination with bacterial recovery experiments, which were different sampling amounts, different sampling sites and different dilution methods. The SPSS software was used to analyze the results of the recovery of bacteria under 3 treatments. Results The 3 methods of experimental design had significant differences in the recovery of bacteria (P<0.05). The pretreatment method for microbial test samples in cucumber and lettuce used 50 g sample volume, and the homogenization method was adopted to achieve the most effective recovery of bacteria. If the sampling site was to maximize the risk of eating, it was recommended to select surface sampling. If the overall pollution level needed to be considered, it was recommended to sample the surface and interior of the sample as a whole. Conclusion The improved pretreatment method of fresh fruit and vegetable can more effectively recover the bacteria in the fruit and vegetable samples, and provides effective suggestions for formulating relevant testing standards or pre-treatment methods for fruit and vegetable microbial research samples.
标题：从蔬菜中回收细菌的前处理方法研究
英文标题：Study on pretreatment methods for recovering bacteria from vegetables
作者：
代晓航 四川省农业科学院分析测试中心；农业部农产品质量安全风险评估实验室(成都)
魏超 四川省农业科学院分析测试中心；农业部农产品质量安全风险评估实验室(成都)
中文关键词:蔬菜,细菌,回收,前处理,
英文关键词:vegetables,bacteria,recovering,pretreatment,
发表日期：2019-01-17
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