响应面法优化芫根皂苷超声提取工艺
目的 采用响应面法优化芫根总皂苷超声提取工艺。方法 以芫根皂苷提取率为指标, 在单因素实验基础上运用Box-Behnken响应面法设计以超声时间、提取温度、液料比3因素3水平的提取实验方案, 按方案进行芫根皂苷提取实验, 以响应面分析法(response surface methodology, RSM)对实验数据进行拟合分析, 建立相应的数学回归模型, 并进行方差分析, 分析推导出芫根皂苷超声辅助提取最佳提取条件。结果 超声提取芫根皂苷的最优工艺条件为: 提取温度61.4 ℃, 液料比22.82:1 (m:V), 提取时间35.96 min, 皂苷提取率为0.3691%。为简化操作, 按提取温度61 ℃, 液料比22:1(m:V), 提取时间36 min进行3次平行实验, 芫根皂苷提取率为0.3635%, 与模型预测值0.3691%相差较小。结论 实验所确定的最优条件方便、可行。
Objective To optimize the ultrasonic extraction technology of saponins from Brassica rapa L. by response surface methodology. Methods Taking the extraction rate of saponin as the index, based on the single factor experiment, the Box-Behnken response surface methodology (RSM) was used to design the experimental scheme with 3 factors (ultrasonic time, extraction temperature and liquid-material ratio) and 3 levels. The extraction experiment was carried out according to the scheme. The experimental data was analyzed by RSM, the corresponding mathematical regression model was established, and the variance analysis was carried out. The optimal extraction conditions for ultrasonic extraction of Brassica rapa L. saponins were determined. Results The optimal extraction conditions for ultrasonic extraction of Brassica rapa L. saponins were as follows: extraction temperature was 61.4 ℃, liquid-material ratio was 22.82:1 (m:V), extraction time was 35.96 min, and the extraction rate of saponin was 0.3691%. In order to simplify the operation, 3 parallel experiments were carried out according to the extraction temperature of 61 ℃, the liquid-material ratio of 22:1 (m:V), and the extraction time of 36 min, the extraction rate of saponin was 0.3635%, which was less than the model prediction value of 0.3691%. Conclusion This optimal conditions determined by the experiment are convenient and feasible.
标题：响应面法优化芫根皂苷超声提取工艺
英文标题：Optimization of ultrasonic extraction technology of saponins from Brassica rapa L. by response surface methodology
作者：
李古兵 西南交通大学医学院/西南交通大学附属成都市第三人民医院
谭秀娟 西南交通大学医学院/西南交通大学附属成都市第三人民医院
何庆 西南交通大学医学院/西南交通大学附属成都市第三人民医院
中文关键词:芫根,皂苷,超声提取,响应面分析法,
英文关键词:Brassica rapa L.,saponins,ultrasonic extraction,response surface methodology,
发表日期：2019-03-15
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