β-环糊精对桔青霉素荧光增强作用研究

β-环糊精对桔青霉素荧光增强作用研究
目的  研究β-环糊精(β-cyclodextrin, β-CD)对桔青霉素(citrinin, CIT)荧光的增强效果。方法  在乙酸、磷酸和β-CD不同浓度下, 分别扫描CIT的荧光发射光谱, 分析其最大发射波长及其荧光强度的变化规律, 利用Benesi-Hildebrand公式计算分析CIT和β-CD结合情况。结果  乙酸或磷酸提供[H+]浓度的大小与CIT的荧光强度高低呈正相关性, 其次乙酸与CIT发生加成反应生成复合物进一步增加CIT的荧光强度。CIT进入β-CD的疏水腔后, 非辐射能量的损失减少, 同时增强CIT的刚性平面结构, 提高CIT的荧光强度。通过Benesi-Hildebrand公式计算分析, CIT/β-CD是以摩尔比1:1形成复合物, 该复合物在磷酸体系下结合常数K接近于恒定值200, 在乙酸体系下随[H+]浓度的增加而减小。结论  阐述了CIT在多种条件下的荧光特性, 为建立CIT的荧光测试方法提供理论依据。

Objective  To study the enhancement effect of β-cyclodextrin (β-CD) on the fluorescence of citrinin (CIT). Methods  The fluorescence emission spectra of CIT were scanned at different concentrations of acetic acid, phosphoric acid and beta-cyclodextrin (β-CD). The maximum emission wavelength and fluorescence intensity of CIT were analyzed. The binding situation of CIT and β-CD was calculated and analyzed by using Benesi-Hildebrand formula. Results  The concentration of [H+] supplied by acetic acid or phosphoric acid was positively correlated with the fluorescence intensity of CIT, and the addition reaction of acetic acid and CIT further increased the fluorescence intensity of CIT. When CIT entered the hydrophobic cavity of β-CD, the loss of non-radiative energy decreased, and the rigid plane structure of CIT was enhanced to enhance the fluorescence intensity of CIT. According to Benesi-Hildebrand formula calculation and analysis, CIT/β-CD was a complex with molar ratio of 1:1. The binding constant K of the complex in phosphoric acid system was close to the constant value of 200, and decreased with the  increase of [H+] concentration in acetic acid system. Conclusion  The fluorescence characteristics of CIT under various conditions are described, which provided a theoretical basis for establishing the fluorescence test method of CIT.

标题：β-环糊精对桔青霉素荧光增强作用研究
英文标题：Study on the enhancement effect of β-cyclodextrin on the fluorescence intensity of citrinin

作者：
陈建彪 通标标准技术服务(上海)有限公司

中文关键词:桔青霉素,β-环糊精,荧光增强,
英文关键词:citrinin,β-cyclodextrin,fluorescence enhancement,

发表日期：2019-03-13

2025-2-2 20:03 上传 β-环糊精对桔青霉素荧光增强作用研究.pdf

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