Objective To develop a method for the rapid determination of total malachite green (MG) residues in aquatic products by colloidal gold immunochromatography assay (GICA). Methods Colloidal gold was prepared by sodium citrate reduction method, which was marked malachite green monoclonal antibody. Under optimized conditions, a sensitive GICA was developed based on competitive binding immunoassay. Results The limit of detection was 2 μg/mL for total amount of leucomalachite green (LMG) residues in aquatic products, and the cross-reaction with the structural analog crystal violet was not obvious. The difference between the actual sample test results and the liquid chromatography-mass spectrometry results was small. Conclusion This method is rapid and efficient, which can meet the need of on-site rapid detection of LMG and MG residues in aquatic products.
标题：胶体金免疫层析法检测水产品中孔雀石绿总量残留
英文标题：Determination of total malachite green residues in aquatic products by colloidal gold immunochromatographic assay
