Objective To identify glycosidic bond characteristic of disaccharide and trisaccharide isomers in galactooligosaccharide(GOS) and isomer fractions isolated from the GOS by hydrophilic interaction liquid chromatography-tandem ion trap mass spectrometry (HILIC-MSn). Methods Relative abundance for characteristic m/z of neutral loss ions from disaccharide and trisaccharide had been acquired by MS2 and MS3 analysis and compared with relevant data in the literature. Results The disaccharide and trisaccharide isomer composition and glycosidic bond characteristics of the quantum high-tech GOS products were preliminarily determined. The relative content of every disaccharide and trisaccharide isomer of GOS was calculated based on the relative percentage of different polymerization degree oligosaccharides measured by HPLC with differential refractive index detector and the relative proportion of each isomer peak height. Conclusion This method is simple and rapid, and can be used to identify and characterize GOS products from different sources, which is expected to solve the problem of qualitative and quantitative analysis of GOS in complex food substrates such as dairy beverages.
标题：基于亲水相互作用色谱-串联离子阱质谱法分析的低聚半乳糖异构体糖苷键的初步鉴定
英文标题：Preliminary analysis of glycosidic bond of galactooligosaccharide isomers by hydrophilic interaction liquid chromatography-tandem ion trap mass spectrometry
中文关键词:低聚半乳糖,异构体,糖苷键,亲水相互作用色谱法,串联离子阱质谱法,
英文关键词:galactooligosaccharides,isomers,glycosidic bond,hydrophilic interaction liquid chromatography,tandem ion trap mass spectrometry,
