固相萃取-超高效液相色谱-串联质谱法快速测定鸡蛋和鸡肉中的7种性激素药物残留量
目的 建立同时测定鸡蛋和鸡肉中7种性激素类药物残留的快速检测方法。方法 鸡蛋和鸡肉中的性激素类药物经乙腈-水溶液(80:20, V:V)提取后, Oasis PRiME HLB固相萃取净化, UPLC-MS/MS法检测, 基质匹配外标法定量。结果 该方法具有良好的准确度和精密度, 在鸡蛋和鸡肉中添加回收率范围分别为78.7%~101.7%和77.9%~104.3%, 相对标准偏差(relative standard deviation, RSD)低于8.7%, 检出限(limit of detection, LOD, S/N=3)和定量限(limit of quantitation, LOQ, S/N=10)分别为0.02~0.5 μg/kg和0.08~2.0 μg/kg。结论 本方法简便快速, 灵敏可靠, 适用于鸡蛋和鸡肉中性激素类药物残留高通量快速检测分析。
Objective To establish a method for the simultaneous determination of 7 kinds of hormones residues in eggs and chicken. Methods The hormones residues in eggs and chicken were extracted by cetonitrile/water (80:20, V:V). The extracts were purified by the Oasis PRiME HLB solid phase extraction, then analyzed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and the matrix-matching external standard method was used to quantify. Results The method had good accuracy and precision, and the recoveries of egg and chicken were 78.7%-101.7% and 77.9%-104.3%, respectively, with the relative standard deviations (RSDs) of lower than 8.7%. The limits of detection (LODs, S/N≥3) and quantification (LOQs, S/N≥10) were 0.02-0.5 μg/kg and 0.8-2.0 μg/kg, respectively. Conclusion The method is simple, fast, sensitive and reliable, which is suitable for the rapid determination of hormones residues in eggs and chicken.
标题：固相萃取-超高效液相色谱-串联质谱法快速测定鸡蛋和鸡肉中的7种性激素药物残留量
英文标题：Rapid determination of 7 kinds of hormones residues in eggs and chicken by solid phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry
作者：
尹伶灵 山东省兽药质量检验所,山东省畜产品质量安全监测与风险评估重点实验室
杨修镇 山东省兽药质量检验所,山东省畜产品质量安全监测与风险评估重点实验室
陈玲 山东省兽药质量检验所,山东省畜产品质量安全监测与风险评估重点实验室
魏秀丽 山东省兽药质量检验所,山东省畜产品质量安全监测与风险评估重点实验室
刘少宁 山东省兽药质量检验所,山东省畜产品质量安全监测与风险评估重点实验室
牛华星 山东省兽药质量检验所,山东省畜产品质量安全监测与风险评估重点实验室
李有志 山东省兽药质量检验所,山东省畜产品质量安全监测与风险评估重点实验室
中文关键词:Oasis PRIME HLB固相萃柱,超高效液相色谱-串联质谱法,性激素残留,鸡蛋,鸡肉,
英文关键词:Oasis PRIME HLB solid phase extraction column,ultra performance liquid chromatography- tandem mass spectrometry,hormones residues,egg,chicken,
发表日期：2019-03-27
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