低聚半乳糖合成研究现状与产业的发展趋势
低聚半乳糖是一种功能性低聚糖, 具有卓越的生理功能, 作为一种新型的食品营养强化剂, 近年来逐渐成为国内外学者研究的热点。根据国内外近二十年的研究资料, 本文综述了以乳糖为底物, 利用β-半乳糖苷酶合成低聚半乳糖的反应机制; 讨论了乳糖底物的初始浓度、反应体系中的水活度、反应温度、反应pH、抑制物(半乳糖、葡萄糖、低聚半乳糖产物)、反应时间等因素对低聚半乳糖产率的具体影响; 总结了不同来源的β-半乳糖苷酶的类型、最佳反应条件下低聚半乳糖的产率, 工业化低聚半乳糖的生产工艺流程; 并对目前市场上销售的商用低聚半乳糖产品及其成分进行了介绍, 探讨了目前制约低聚半乳糖产业在我国大规模发展的因素, 以期促进我国低聚半乳糖产业的发展。
Galactooligosaccharide is a kind of functional oligosaccharide with excellent physiological function. As a new type of food nutrition fortifier, galactooligosaccharide has gradually become a research hotspot of domestic and foreign scholars in recent years. According to the research data of the past 20 years at home and abroad, this paper reviewed the reaction mechanism of the synthesis of galacto-oligosaccharides by β-galactosidase using lactose as substrate, discussed the effects of initial concentration of lactose substrate, water activity in the reaction system, reaction temperature, reaction pH, inhibitor (galactose, glucose, oligogalactose product) and reaction time on the yield of oligogalactose, summarized the types of β-galactosidase from different sources, the yield of galacto-oligosaccharides under optimal reaction conditions, and the production process of industrial galacto-oligosaccharides, introduced the commercial oligogalactose products and their components on the market, and discussed the factors restricting the large-scale development of oligogalactose industry in China, in order to promote the development of galactose industry in China.
标题：低聚半乳糖合成研究现状与产业的发展趋势
英文标题：Research status and development trend of galacto-oligosaccharide synthesis
作者：
张金秋 江苏省食品药品监督检验研究院
王少伟 江苏省食品药品监督检验研究院
陶飞 江苏省食品药品监督检验研究院
徐春祥 江苏省食品药品监督检验研究院
林慧 江苏省食品药品监督检验研究院;中国药科大学食品质量与安全科研室
中文关键词:低聚半乳糖,β-半乳糖苷酶,产率影响因素,酶法合成,
英文关键词:galacto-oligosaccharide,β-galactosidase,yield factors,enzymatic synthesis,
发表日期：2019-03-11
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