生物酶法提取铜藻中褐藻多酚的工艺优化
目的 优化生物酶法破壁提取铜藻中褐藻多酚方法, 提高褐藻多酚的提取率。方法 以铜藻为原料, 生物酶法提取多酚, 通过单因素研究了纤维素酶添加量、酶解温度、酶解时间、酶解pH及底物浓度对多酚提取效果的影响, 并采用正交试验优化提取工艺条件。结果 各因素对多酚得率的影响大小为: 酶解pH>酶解温度>酶添加量>酶解时间; 最佳酶解条件为: 液料比25:1 (mL/g), 酶用量占铜藻粉末质量的4%(酶活力为6000 U), 酶解温度55 ℃, 酶解时间6 h, 酶解pH 5.0。该条件下, 多酚得率达14.74 mg GA/g。结论 与传统提取工艺相比, 生物酶法更加绿色安全且提取效率升高, 可以更广泛的被应用于提取技术当中。
Objective To optimize the method of extracting brown algal polyphenols from Sargassum horneri by enzymatic method, and improve the extraction rate of polyphenols from brown algae. Methods The polyphenols were extracted from Sargassum horneri by enzymatic method. The effects of cellulase addition, enzymatic hydrolysis temperature, enzymatic hydrolysis time, enzymatic hydrolysis pH and substrate concentration on the extraction of polyphenols were studied by single factor tests. The orthogonal test was used to optimize the extraction process conditions. Results The effects of various factors on the yield of polyphenols were as follows: enzymatic pH>enzymatic temperature>enzyme addition>enzymatic time. The optimal enzymatic conditions were as follows: liquid to material ratio was 25:1 (mL/g), the amount of enzyme accounted for 4% of the quality of copperalga powder (enzyme activity: 6000 U), the enzymatic hydrolysis temperature was 55 ℃, the enzymatic hydrolysis time was 6 h, and the enzymatic hydrolysis pH was 5.0. Under this condition, the polyphenol yield was 14.74 mg GA/g. Conclusion Compared with the traditional extraction process, the biological enzymatic method is more green and safe, and the extraction efficiency is higher, which can be widely used in the extraction technology.
标题：生物酶法提取铜藻中褐藻多酚的工艺优化
英文标题：Optimization of extraction process of phlorotannins from Sargassum horneri by enzymatic hydrolysis
作者：
孙露川阳 大连海洋大学食品科学与工程学院
任丹丹 大连海洋大学食品科学与工程学院
王帅 大连海洋大学食品科学与工程学院
吴哲 大连海洋大学食品科学与工程学院
王思元 大连海洋大学食品科学与工程学院
王惜童 大连海洋大学食品科学与工程学院
中文关键词:铜藻,褐藻多酚,酶解法,提取工艺,
英文关键词:Sargassum horneri,phlorotannins,enzymatic hydrolysis,extraction process,
发表日期：2019-02-19
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