高效液相色谱法测定果汁、果酱中的皂黄
目的 建立高效液相色谱法检测果汁、果酱中皂黄含量的分析方法。方法 试样经20%(V:V)甲醇溶液超声提取, 离心取上清液, 高效液相色谱—二极管阵列检测器分析。结果 皂黄在0.50~100 μg/mL浓度范围内呈现良好的线性关系(r2＞0.9999), 具有较高的分离度且峰型对称, 果汁和果酱的标准物质加入实验的回收率分别在96.1%~101.9%和79.2%~99.4%之间, 相对标准偏差(relative standard deviation, RSD)分别为1.1%~2.3%和4.3%~7.4%。结论 该方法具有操作简便、准确度高、灵敏度高和稳定性好等优点, 适用于果汁、果酱中皂黄含量的测定。
Objective To establish a method for the determination of the content of metanil yellow in juice and jam by high performance liquid chromatography (HPLC). Methods The samples were ultrasonically extracted in 20% (V:V) methanol solution, the supernatant after centrifuging was analyzed by high performance liquid chromatography-diode array detector (HPLC-DAD). Results Metanil yellow had good linear relationships in the range of 0.50-100 μg/mL, r2>0.9999. The recoveries of metanil yellow in juice and jam samples were 96.1%-101.9% and 79.2%-99.4%, respectively, with the relative standard deviations of 1.1%-2.3% and 4.3%-7.4%. Conclusion This method had the advantages of easy operating, high sensitivity, high accuracy and good stability, which is suitable for the detection of metanil yellow in juice and jam.
标题：高效液相色谱法测定果汁、果酱中的皂黄
英文标题：Determination of metanil yellow in juice and jam by high performance liquid chromatography
作者：
房艳 谱尼测试集团股份有限公司
高俊海 谱尼测试集团股份有限公司
张雅莉 谱尼测试集团股份有限公司
宋薇 谱尼测试集团股份有限公司
中文关键词:果汁,果酱,皂黄,高效液相色谱法,
英文关键词:juice,jam,metanil yellow,high performance liquid chromatography,
发表日期：2018-10-29
- 文件大小:
- 4.38 MB
- 下载次数:
- 60
-
高速下载
|
|
