化妆品中23种香料类致敏成分的高效液相色谱法测定
建立了同时测定化妆品中23种香料致敏成分的高效液相色谱法。样品经乙腈超声提取15min,以8000r/min离心5min,取上清液过滤后测定。采用Aglient ZORBAX SB-C18(4.6×250 mm,5μm)色谱柱, 以乙腈-水为流动相梯度洗脱,采用二极管阵列检测器多波长检测(200、210、240、290nm)。23种致敏成分的定量限分别为1.25、2.5、5.0、10.0μg/kg,线性范围内相关系数均大于0.99。在1倍定量限、2倍定量限,10倍定量限浓度的添加水平下平均回收率为80%-110%,相对标准偏差为0.5%-9.3%。该方法简单、准确、快速,适用于化妆品中23种致敏成分的测定。
A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of 23 perfume allergens in cosmetics. The cosmetic samples were extracted supersonically with acetonitrile for 15 minutes and centrifuged at 8000 r/min for 5min. The supernatant was separated on an Aglient Zorbax SB-C18 column (4.6×250mm, 5μm) by gradient elution with acetonitrile-water. The analysis of 23 allergens was performed by HPLC with diode array detector at the wavelength of 200, 210, 240, 290 nm, respectively. The limits of quantitation of 23 allergens were 1.25、2.5、5.0、10.0 μg/kg. The method showed a good linearity with correlation coefficients above 0.99. The mean recoveries at three spiked levels were in the range of 80% - 110% with RSDs of 0.5% - 9.3%. The method is accurate, rapid and suitable for the determination of 23 perfume allergens in cosmetics samples.
标题：化妆品中23种香料类致敏成分的高效液相色谱法测定
英文标题：Determination of 23 perfume allergens in cosmetics by high performance liquid chromatography
作者：
李兆杰 威海出入境检验检疫局检验检疫技术中心
吕春莹 威海出入境检验检疫局检验检疫技术中心
鞠玲燕 威海出入境检验检疫局检验检疫技术中心
石桦 海军青岛特勤疗养中心
张慧敏 威海海洋职业学院
崔嘉 威海出入境检验检疫局检验检疫技术中心
徐成刚 威海出入境检验检疫局检验检疫技术中心
中文关键词:化妆品,香料,致敏成分,高效液相色谱,测定,
英文关键词:cosmetics,perfume,allergens,high performance liquid chromatography (HPLC),determination,
发表日期：2018-12-19
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