黑咖啡提取物对人皮肤成纤维细胞胶原蛋白及衰老相关基因的影响
目的 研究黑咖啡提取物对人皮肤成纤维细胞(HSF)胶原蛋白合成及衰老相关基因的影响。方法 体外培养HSF细胞,通过CCK-8法筛选黑咖啡提取物安全剂量;实验分为样品组和空白对照组,用ELISA技术检测黑咖啡提取物对细胞中Ⅰ型胶原蛋白(collagen Ⅰ,COI Ⅰ)和基质金属蛋白酶-1(MMP-1)含量的影响,用RT-PCR技术检测黑咖啡提取物作用后细胞中COI Ⅰ和MMP-1 mRNA的表达水平。结果 与空白对照组比较,黑咖啡提取物可抑制HFF-1细胞中MMP-1合成(P<0.05),促进COI Ⅰ合成(P<0.05);使COI Ⅰ mRNA表达水平升高(P<0.01),而MMP-1 mRNA表达水平降低(P<0.01)。结论 黑咖啡提取物通过促进CoI I和抑制MMP-1表达,对胶原蛋白代谢具有改善和调节作用,可延缓皮肤衰老。
Objective To research the effects of black coffee extract on collagen synthesis of human dermal fibroblasts (HSF)cell and the aging-related genes. Methods Cultured HSF cells in vitro, and screening safe dose of black coffee extract by CCK-8 method . The experiment was divided into experimental group and control group. The effect of black coffee extract on the content of type I collagen(COI I) and matrix metalloproteinase-1(MMP-1)in HSF cells was detected by ELISA methed. The expression in mRNA levels of COI I and MMP-1 about black coffee extract were detected through RT-PCR technology.Result Compared with the control group, black coffee extract could inhibit the synthesis of MMP-1 in HSF cells (P<0.05), promote the synthesis of COI I (P<0.05), and increase the expression of COI I mRNA (P<0.01). And the expression level of MMP-1 mRNA was decreased (P<0.01). Conclusion Black coffee extract can improve and regulate collagen metabolism by promoting CoI I and inhibiting MMP-1 expression, and has the effect of inhibiting skin aging.
标题:黑咖啡提取物对人皮肤成纤维细胞胶原蛋白及衰老相关基因的影响
英文标题:Effects of black coffee extract on collagen and aging related genes in human skin fibroblasts cellCui Liwen1,2,Zhang Jialing1
作者:
崔力文 山西医科大学公共卫生学院
张加玲 山西医科大学公共卫生学院
中文关键词:黑咖啡,人皮肤成纤维细胞,Ⅰ型胶原蛋白,基质金属蛋白酶-1,
英文关键词:black coffee,human skin fibroblasts,type I collagen,matrix metalloproteinase-1,
发表日期:2018-12-05
- 文件大小:
- 4.69 MB
- 下载次数:
- 60
-
高速下载
|
|