黑咖啡提取物对人皮肤成纤维细胞胶原蛋白及衰老相关基因的影响
目的 研究黑咖啡提取物对人皮肤成纤维细胞(HSF)胶原蛋白合成及衰老相关基因的影响。方法 体外培养HSF细胞,通过CCK-8法筛选黑咖啡提取物安全剂量；实验分为样品组和空白对照组,用ELISA技术检测黑咖啡提取物对细胞中Ⅰ型胶原蛋白(collagen Ⅰ,COI Ⅰ)和基质金属蛋白酶-1(MMP-1)含量的影响,用RT-PCR技术检测黑咖啡提取物作用后细胞中COI Ⅰ和MMP-1 mRNA的表达水平。结果 与空白对照组比较,黑咖啡提取物可抑制HFF-1细胞中MMP-1合成(P<0.05),促进COI Ⅰ合成(P<0.05)；使COI Ⅰ mRNA表达水平升高(P<0.01),而MMP-1 mRNA表达水平降低(P<0.01)。结论 黑咖啡提取物通过促进CoI I和抑制MMP-1表达,对胶原蛋白代谢具有改善和调节作用,可延缓皮肤衰老。
Objective To research the effects of black coffee extract on collagen synthesis of human dermal fibroblasts (HSF)cell and the aging-related genes. Methods Cultured HSF cells in vitro, and screening safe dose of black coffee extract by CCK-8 method . The experiment was divided into experimental group and control group. The effect of black coffee extract on the content of type I collagen(COI I) and matrix metalloproteinase-1(MMP-1)in HSF cells was detected by ELISA methed. The expression in mRNA levels of COI I and MMP-1 about black coffee extract were detected through RT-PCR technology.Result Compared with the control group, black coffee extract could inhibit the synthesis of MMP-1 in HSF cells (P<0.05), promote the synthesis of COI I (P<0.05), and increase the expression of COI I mRNA (P<0.01). And the expression level of MMP-1 mRNA was decreased (P<0.01). Conclusion Black coffee extract can improve and regulate collagen metabolism by promoting CoI I and inhibiting MMP-1 expression, and has the effect of inhibiting skin aging.
标题：黑咖啡提取物对人皮肤成纤维细胞胶原蛋白及衰老相关基因的影响
英文标题：Effects of black coffee extract on collagen and aging related genes in human skin fibroblasts cellCui Liwen1,2,Zhang Jialing1
作者：
崔力文 山西医科大学公共卫生学院
张加玲 山西医科大学公共卫生学院
中文关键词:黑咖啡,人皮肤成纤维细胞,Ⅰ型胶原蛋白,基质金属蛋白酶-1,
英文关键词:black coffee,human skin fibroblasts,type I collagen,matrix metalloproteinase-1,
发表日期：2018-12-05
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