在场拭子法检测猪组织中抗生素残留
摘 要: 目的 建立在场拭子法(swab test on premises, STOP)并对深圳市不同来源的猪肉、猪肝和猪肾抗生素残留进行了筛查。方法 选用枯草芽孢杆菌CMCC-63501作为实验敏感菌株，通过测定猪肉组织的加药回收率、检出限和假阴性率建立STOP法，并对市售猪组织中抗生素残留量进行筛查。结果 建立了乳酸环丙沙星、盐酸多西环素、恩诺沙星、硫酸新霉素、头孢噻呋钠和硫酸庆大霉素共6种敏感抗生素的标准曲线，建立了组织抗生素残留检测的STOP法，加药回收率、检出限和假阴性率均符合方法的要求。采用STOP法对不同来源猪肉、猪肝和猪肾中抗生素残留量的筛查结果表明，猪肝和猪肾阳性超标率分别为24%和10%，这些样本均来源于郊区，其中农贸市场的风险要高于超市，而土猪和外三元的品种之间没有明显差异。结论 猪组织抗生素残留风险高发的部位、区域和场所分别是内脏、郊区和农贸市场，与猪的品种没有明显的相关性。STOP法简单快捷易操作，可适用于动物性食品中抗生素残留的现场检测。
ABSTRACT: Objective To establish a swab test on premises (STOP) method and determine the antibiotic residues in muscle, liver and kidney of pig from different sources in Shenzhen. Methods Bacillus subtilis CMCC-63501 was selected as the experimental sensitive strain. The STOP method was established through the detection of antibiotics spiked recoveries, limit of detection and false negative rate in pork. Antibiotic residues of the pig tissues circulating in the market were screened. Results The standard curves were established for 6 antibiotics including ciprofloxacin lactate, doxycycline hydrochloride, enrofloxacin, neomycin sulfate, ceftiofur sodium and gentamicin sulfate. The STOP method was established for detection of antibiotics in pork. Spiked recoveries, limit of detection and false negative rate was satisfied the requirements of methodology. The STOP method was used to screen antibiotic residues in pork, pig liver and pig kidney from different sources. The results showed that positive over-standard rates of liver and kidney were 24% and 10 % respectively and all of which came from rural areas. Moreover, the risk of farmers' market was higher than that of supermarkets. There was no significant difference between native breed pigs and foreign breed pigs. Conclusion The tissue, region and market of high risk of antibiotic residues in pig tissues are visceral, suburban area and farmers' markets. Antibiotic residues had no significantly correlated with pig breeds. This method is fast and easy to be operated, which might be applicable to the presence detection of antibiotic residues in animal foods.
标题：在场拭子法检测猪组织中抗生素残留
英文标题：Determination of antibiotic residues in pig tissues by swab test on premises
作者：
黄林丽 深圳大学化学与环境工程学院食品科学与工程系
欧阳子程 深圳大学化学与环境工程学院食品科学与工程系
魏焘 深圳大学化学与环境工程学院食品科学与工程系
王梓莹 深圳大学化学与环境工程学院食品科学与工程系
黄思莹 深圳大学化学与环境工程学院食品科学与工程系
张悦彤 深圳大学化学与环境工程学院食品科学与工程系
蔡文静 深圳大学化学与环境工程学院食品科学与工程系
孙玉立 深圳大学化学与环境工程学院食品科学与工程系
邓嘉敏 深圳大学化学与环境工程学院食品科学与工程系
李铁军 中国科学院亚热带农业生态研究所
何庆华 深圳大学化学与环境工程学院食品科学与工程系;中国科学院亚热带农业生态研究所
中文关键词:猪组织,抗生素,残留,微生物方法,
英文关键词:pig tissues,antibiotic,residues,microbiological methods,
发表日期：2018-10-09
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