3种不同培养基和1种测试片检测水溶性化妆品中菌落总数的结果分析

3种不同培养基和1种测试片检测水溶性化妆品中菌落总数的结果分析
目的  使用t检验法分析3种不同培养基和1种测试片检测水溶性化妆品中菌落总数结果。方法  参照《化妆品安全技术规范》(2015年版), 使用卵磷脂吐温80营养琼脂、TTC营养琼脂、平板计数琼脂方法进行样品菌落总数检测, 然后使用PetrifilmTM菌落测试片检测样品。运用配对t检验法评价4种不同方法对水溶性化妆品中菌落总数计数结果一致性的影响。结果  在102、103 CFU/mL 2种不同浓度, 平板计数琼脂法结果波动性最大, 无法抑制菌落蔓延, 而 TTC营养琼脂和菌落测试片相对卵磷脂吐温80营养琼脂, 具有抑制菌落蔓延生长、提高计数结果准确性的作用。结论  在一定浓度下, 可根据实际情况来选择相应培养基检测水溶性化妆品中菌落总数以提高检测效率和质量。

Objective  To analyze the total number of bacterial colonies in water-soluble cosmetics used 3 different media and PetrifilmTM by t test. Methods  According to the Safety and technical standards for cosmetics (2015 Edition), the total number of bacterial colonies in sample was detected by the methods of lecithin Twain 80 Nutrient Agar, TTC nutrient agar and plate counting agar, respectively. The samples were also detected by PetrifilmTM colony test. Paired t test was used to evaluate the effect of 4 different methods on the consistency of count of bacterial count in water-soluble cosmetics. Results  In 2 different concentrations of 102 and 103 CFU/mL, the results of plate counting agar method showed the greatest fluctuation, which could not inhibit colony spread. While TTC nutrient agar and colony test tablets, which were compared to lecithin Twain 80 nutrient agar, could inhibit the growth of bacterial colonies. Thus they improved the accuracy of counting results. Conclusion  Under certain concentration, the corresponding medium can be selected according to the actual situation to detect the total number of bacterial colonies in water-soluble cosmetics, so as to improve the detection efficiency and quality.

标题：3种不同培养基和1种测试片检测水溶性化妆品中菌落总数的结果分析
英文标题：Comparison of 3 different media and PetrifilmTM for detection of total bacterial count in water-soluble cosmetics

作者：
黄玲玲 广东产品质量监督检验研究院广东顺德528000
刘辉 广东产品质量监督检验研究院
黄宝莹 广东产品质量监督检验研究院广东顺德528000
赵玲 广东产品质量监督检验研究院广东顺德528000
杨纯佳 广东产品质量监督检验研究院广东顺德528000
周臣清 广东产品质量监督检验研究院广东顺德528000
严家俊 广东产品质量监督检验研究院广东顺德528000

中文关键词:化妆品,菌落总数,计数,配对t检验,
英文关键词:cosmetics,total number of colonies,counting,paired t test,

发表日期：2018-03-23

2025-1-31 20:04 上传 3种不同培养基和1种测试片检测水溶性化妆品中菌落总数的结果分析.pdf

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