响应面法优化小春花根黄酮提取工艺及其抗油脂氧化活性研究
目的 研究小春花根黄酮提取工艺及其抗油脂氧化活性。方法 以总黄酮提取率为考察指标, 利用单因素试验结合响应面Box－Benhnken试验设计方法, 研究乙醇浓度、料液比、提取时间、提取温度4个因素对提取工艺的影响, 优化小春花根黄酮提取工艺; 通过烘箱热贮藏实验研究小春花根黄酮对油脂的抗氧化作用。结果 影响小春花根总黄酮提取的因素依次为: 超声提取温度＞乙醇浓度＞提取时间＞料液比, 小春花根总黄酮最佳工艺条件为: 乙醇体积分数73%, 料液比1:43(g/mL), 提取时间73 min, 提取温度71 ℃。黄酮提取得率为3.4299 mg/g, 与理论预测值3.4913 mg/g接近。抗氧化活性分析表明, 黄酮提取物有较强抗油脂氧化活性; 对油脂的抗氧化能力与黄酮提取物浓度成正相关, 1 mg/mL黄酮提取物与VC的抗氧化作用相当。 结论 小春花根黄酮对油脂良好的保护效果, 在油脂抗氧化添加剂的应用方面具有潜在的开发价值。
Objective To investigate the best extraction process of total flavonoids from Sceptridium ternatum (thumb.) Lyon root, and analyze the antioxidant activities on oil of total flavonoids. Methods The extraction rate of total flavonoids was used as an index, and the effects of four factors on the extraction process were studied by single factor test and response surface Box-Benhnken test, including ethanol concentration, ratio of material to liquid, extraction time and extraction temperature. Flavonoids extraction process from Sceptridium ternatum (thumb.) Lyon root was optimized. The antioxidation effects of flavonoids from Sceptridium ternatum (thumb.) Lyon root on oil and fat were studied by using the evaluation of oven strengthen storage experiment. Results The factors influencing the extraction of total flavonoids from Sceptridium ternatum (thumb.) Lyon root were ultrasonic extraction temperature>ethanol concentration>extraction time>the ratio of material to liquid. The optimum technological conditions of extraction of total flavonoids from Sceptridium ternatum (thumb.) Lyon root were as follows: ethanol volume fraction was 73%, ratio of material to liquid was 1:43 (g/mL), extraction time was 73 min, and extraction temperature was 71 ℃. The extraction yield of flavonoids was 3.4299 mg/g, which was close to the theoretical prediction value of 3.4913 mg/g. The antioxidant activity analysis showed that the flavonoid extract had strong anti-lipid oxidation activity. The antioxidant activity of flavonoid extract to oil was positively correlated with the concentration of flavonoid extract. The antioxidant activity of 1 mg/mL flavonoid extract was similar to that of VC. Conclusion Flavonoids from Sceptridium ternatum (thumb.) Lyon root have good protective effects on oil and fat, and have potential development values in the application of antioxidation additive of oil and fat.
标题：响应面法优化小春花根黄酮提取工艺及其抗油脂氧化活性研究
英文标题：Optimization of extraction process of flavonoids from Sceptridium ternatum (thumb.) Lyon root using response surface methodology and evaluation of its antioxidant activity
作者：
徐连巧 贵州师范学院化学与生命科学学院
曹剑锋 贵州师范学院化学与生命科学学院,贵州师范学院药用植物生物技术研究所,贵州省茶籽资源综合利用工程研究中心
谢之英 贵州师范学院化学与生命科学学院
王自布 贵州师范学院化学与生命科学学院,贵州师范学院药用植物生物技术研究所
何军 贵州师范学院化学与生命科学学院
陈集双 南京工业大学生物与制药工程学院
朱锡健 贵州师范学院化学与生命科学学院
中文关键词:小春花根,黄酮,响应面,抗氧化,
英文关键词:Sceptridium ternatum(thumb.) LyonSroot,flavonoids,response surface methodology,antioxidation,
发表日期：2018-01-18
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