H2O2-甲基红催化光度法测定亚硒酸钠片中硒的含量
目的 基于在HCl介质中硒对H2O2氧化甲基红有较强的催化作用, 建立了测定亚硒酸钠片中硒含量的新方法。方法 在2支具塞比色管中, 各依次准确加入甲基红溶液2.0 mL(300 μg/mL)、H2O2溶液2.5 mL (3%)、盐酸溶液2.0 mL(1 mol/L), 于其中1支比色管加入一系列不同浓度的硒标准溶液, 另1支作试剂空白。在80 ℃水浴10 min, 冷却后, 用1 cm比色皿在波长518 nm处, 以蒸馏水为为参比, 分别测定测定其ΔA。 结果 硒在0.2~1.2 μg/mL之间存在线性关系, 线性方程为ΔA=0.263C+0.0117 (r2=0.9988), 检出限为 0.186 μg/mL, 表征摩尔吸收系数为2.35×105 L/(mol?cm)。结论 将该方法用于亚硒酸钠片中硒的含量测定, 与2,3-二氨萘荧光法比较结果准确可靠、操作简便。
Objective To establish a new method for the determination of selenium in sodium selenite tablets based on the strong catalytic effect of selenium on the oxidation of methyl red by H2O2 in HCl medium. Methods Totally 2.0 mL (300 μg/mL) of methyl red solution, 2.5 mL (3%) of H2O2 solution and 2.0 mL (1 mol/L) of hydrochloric acid solution were added into each of two plugged colorimetric tubes. One colorimetric tube was added a series of different concentrations of selenium standard solution, with the other as a reagent blank. After cooled at 80 ℃ for 10 min, the linearity of ΔA was determined at a wavelength of 518 nm with distilled water as the reference. Results Selenium has linear relationship in the range of 0.2~1.2 μg/mL. The linear equation was ΔA=0.263C+0.0117 (r2=0.9988). The limit of detection was 0.186 μg/mL. The molar absorption coefficient was 2.35×105 L/(mol?cm). Conclusion Comparison with 2,3-diamine fluorescence method, this method is accurate, reliable and easy to operate, which can be used to determine the content of selenium in sodium selenite tablets.
标题：H2O2-甲基红催化光度法测定亚硒酸钠片中硒的含量
英文标题：Determination of selenium in sodium selenite tablets by H2O2-methyl red catalytic spectrophotometry
作者：
白静 河南应用技术职业学院化学工程学院
崔丽伟 河南牧业经济学院食品工程学院
中文关键词:硒,催化光度法,H2O2-甲基红；亚硒酸钠片,
英文关键词:selenium,catalytic spectrophotometry,H2O2-methyl red,Sodium selenite tablets,
发表日期：2017-06-08
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