Objective To evaluate the consistency of the determination of foodborne pathogenic microorganisms such as Enterobacter sakazakii and Listeria monocytogenes by constant temperature fluorescent nucleic acid detector, with the traditional national standard culture method as a reference alignment method. Methods The sensitivity, specificity, false positive rate, false negative rate and accuracy of constant temperature fluorescent nucleic acid detector for Enterobacter sakazakii and Listeria monocytogenes were investigated with the national standards method as reference. Results There were no false negative and misdetection rate. Both the sensitivities and specificities of Enterobacter sakazakii and Listeria monocytogenes detected by the constant temperature fluorescent nucleic acid detector were 100%. Conclusion The constant temperature fluorescent nucleic acid detector method has the advantages of high specificity and accuracy, and has no missing detection.
标题：恒温荧光核酸检测仪检测2种食源性致病菌的方法验证
英文标题：Method verification for the detection of 2 kinds of food pathogens by constant temperature fluorescent nucleic acid detector
