肉及肉制品牛源性成分核酸检测试剂盒评价技术的研究
目的 探索适合评价核酸检测试剂盒的评价方法。方法 本研究采用室内技术参数验证的方法对某公司的肉及肉制品牛源性成分核酸检测试剂盒(实时荧光PCR法)进行评价。对检测的灵敏度特异性、假阳性/假阴性、检测限、耐受性及与标准方法的一致性共6个方面的参数分析评价。结果 评价结果表明, 对生鲜肉、酱卤肉、熏烤肉、速冻调理肉制品4类共40种肉制品, 试剂盒检测结果与标准检测方法检测结果无显著差异, 即试剂盒可以满足肉及肉制品牛源性成分核酸检测的需求。结论 本文为检测机构筛选合适的牛源性成分检测试剂盒有一定的借鉴意义, 并对建立核酸检测试剂盒的评价方法提供一定的技术帮助。
Objective To explore a suitable method for the evaluation of nucleic acid detection kit. Methods The meat and meat products nucleic acid detection kit (real-time PCR method) from biological composition was evaluated by the method of indoor technology parameter validation. Total of 6 parameters about the sensitivity, the specificity, and the false positives/false negatives, detection limit, tolerance and conformance with the standard method were analyzed and evaluated. Results There was no significant difference between the kit method and the reference method for 40 kinds of meat products about raw meat, pot-stewed meat, smoked meat, quick-frozen meat, which meant the kit method could meet our requirement for meet and meet product nucleic acid detection. Conclusion It has certain reference significance for screening institutions to screen suitable bovine source components detection kit, and provide some technical help for establishing the evaluation method of nucleic acid detection kit.
标题：肉及肉制品牛源性成分核酸检测试剂盒评价技术的研究
英文标题：Evaluation of detection of bovine derived nucleic acid detection kit for meat and meat products
作者：
石盼盼 河南省产品质量监督检验院食品中心
谢文佳 河南省产品质量监督检验院食品中心
魏法山 河南省产品质量监督检验院食品中心
中文关键词:肉及肉制品,核酸检测试剂盒,实时荧光PCR法,评价,
英文关键词:meet and meet product,nucleic acid detection kit,real-time PCR method,evaluation,
发表日期：2017-11-28
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