液相色谱元素分析仪-同位素质谱法鉴定蜂蜜 掺假的方法改进

液相色谱元素分析仪-同位素质谱法鉴定蜂蜜 掺假的方法改进
目的  改进液相色谱/元素分析仪-同位素质谱法鉴别蜂蜜掺假的方法。方法  对现有欧盟标准方法优化液相色谱条件, 结合元素分析仪-同位素质谱法, 将二糖分离为麦芽糖、蔗糖, 提出一个新的参数—麦芽糖、蔗糖δ13C值之差δ13CM-S。结果  根据本研究检测113个国内外不同来源纯正蜂蜜样本的数据, 提出纯正蜂蜜δ13C值新要求: 蜂蜜蛋白质与蜂蜜同位素差值δ13CP-H大于?0.97‰; 果糖、葡萄糖δ13C值之差δ13CF-G在?0.60‰至0.56‰范围内; 麦芽糖、蔗糖δ13C值之差δ13CM-S在?0.73‰至0.98‰范围内; 各个组分δ13C最大差值δ13Cmax小于2.05‰; 根据上述4个参数来确认蜂蜜是否掺假。在日常检测和市场销售的160个样品中, 原方法阳性检出率为16.2%, 而新方法阳性检出率达21.9%。结论  本研究提升了蜂蜜掺假检测能力, 此方法的建立更好、更精确打击掺假的同时, 也维护消费者权益。

Objective  To optimize the method for identification of honey adulteration by liquid chromatography/elementary analysis-isotope ratio mass spectrometry. Methods  According to EU standard methods, the liquid chromatography conditions were optimized and element analyzer-isotopic mass spectrometry were combined. Disaccharide was separated into maltose and sucrose. A new parameter, δ13C difference between maltose and sucrose (δ13CM-S), was presented. Results  Based on the isotopic data of 113 authentic honeys from different sources at home and abroad, the δ13C value limits for the authentic honeys were proposed: δ13C difference between honey and protein (δ13CP-H) was more than ?0.97‰, δ13C difference between fructose and glucose (δ13CF-G) was from ?0.60‰ to 0.56‰, δ13CM-S was from ?0.73‰ to 0.98‰ and the maximum difference between all δ13C values should be less than 2.05‰ (δ13Cmax<2.05‰). Under the pure honey criteria, the positive percentage by the new method was 21.9% for 160 commercial samples, while the positive percentage by the old method was only 16.2%. Conclusion  This study improves the detection ability of honey adulteration, which is better and more accurate to combat adulteration, while maintaining the rights and interests of consumers.

标题：液相色谱元素分析仪-同位素质谱法鉴定蜂蜜 掺假的方法改进
英文标题：Optimization of identification of honey adulteration by liquid chromatography/elementary analysis-isotope ratio mass spectrometry

作者：
季美泉 江苏出入境检验检疫局食品实验室
林宏 江苏出入境检验检疫局食品实验室
丁涛 江苏出入境检验检疫局食品实验室
王艳 江苏出入境检验检疫局食品实验室
桂茜雯 江苏出入境检验检疫局食品实验室
刘芸 江苏出入境检验检疫局食品实验室
张建 江苏出入境检验检疫局食品实验室
费晓庆 江苏出入境检验检疫局食品实验室
谭梦茹 无锡市药品安全检验检测中心
赵梦婷 东南大学成贤学院

中文关键词:蜂蜜掺假,同位素质谱,液相色谱,
英文关键词:honey adulteration,isotope ratio mass spectrometry,liquid chromatography,

发表日期：2017-11-29

2025-1-31 19:53 上传 液相色谱元素分析仪-同位素质谱法鉴定蜂蜜 掺假的方法改进.pdf

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