响应面法优化超声辅助提取黄秋葵花总黄酮的工艺研究
目的 采用响应面法对超声辅助提取黄秋葵花总黄酮的工艺进行优化。方法 在提取剂浓度、液料比例、提取温度、水浴提取时间、超声波提取时间试验结果的基础上, 采用Box-Behnken design试验设计原理, 设计4因素3水平试验, 以响应面分析法优化液料比例、乙醇浓度、提取温度、水浴提取时间4个因素对黄秋葵花总黄酮提取率的影响。结果 黄秋葵花总黄酮提取率的最佳工艺条件为: 液料比例160:1(mL:g)、乙醇浓度42%、提取温度80 ℃、水浴提取时间16 min和超声提取时间30 min。在此条件下, 黄秋葵花总黄酮的提取率为(2.487±0.05)%, 真实值与模型预测值相对误差为1.23%。结论 该提取工艺提高了黄秋葵花总黄酮的提取率, 为其开发利用提供理论支持。
Objective To optimize the ultrasonic-assisted extraction of total flavones from okra flowers by response surface methodology. Methods Based on the results of extraction concentration, ratio of solvent to material, extraction temperature, water bath extraction time and ultrasonic extraction time experiment, the Box-Behnken design method was used to conduct the 4-factors and 3-levels experiment on the basis of single-factor responsive surface analysis, with the yield of total flavones as response value, and the extraction conditions including the ratio of solvent to material, ethanol concentration, extraction temperature and water bath extraction time as response factors. Results The optimal preparation process of ultrasonic extraction for total flavones from okra flowers were as follows: 160:1 (mL:g) of solvent to material, 42% ethanol concentration, 80 ℃ of extraction temperature, 16 min of water bath extraction time and 30 min of ultrasonic extraction time. Under this condition, the extracting rate of total flavones from okra flowers was (2.487±0.05)% and the relative error of real value and model prediction value was 1.23%. Conclusion The extraction process improves the extraction rate of total flavones from okra flowers, which provides theoretical support for its development and utilization.
标题：响应面法优化超声辅助提取黄秋葵花总黄酮的工艺研究
英文标题：Optimization of ultrasonic-assisted extraction of total flavones from okra flowers by response surface methodology
作者：
林香信 农业部农产品质量安全风险评估实验室(福州),福建省农业科学院农业质量标准与检测技术研究所
颜孙安 农业部农产品质量安全风险评估实验室(福州),福建省农业科学院农业质量标准与检测技术研究所
姚清华 农业部农产品质量安全风险评估实验室(福州),福建省农业科学院农业质量标准与检测技术研究所
赖玉林 农业部农产品质量安全风险评估实验室(福州),福建省农业科学院农业质量标准与检测技术研究所
林丽 农业部农产品质量安全风险评估实验室(福州),福建省农业科学院农业质量标准与检测技术研究所
林虬 农业部农产品质量安全风险评估实验室(福州),福建省农业科学院农业质量标准与检测技术研究所
中文关键词:黄秋葵花,总黄酮,超声提取,响应面分析法,
英文关键词:Okra flowers,Total flavones,Ultrasonic extraction,Response surface methodology,
发表日期：2017-12-19
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