Objective To establish a method for rapid determination of Yersinia pseudotuberculosis in food based on PCR-nucleic acid test strip technology. Methods Ten strains of Yersinia pseudotuberculosis, 9 other strains of Yersinia enterocolitica and 18 strains of source strains were used as test strains for specific tests. The sensitivity was verified by counting pure bacterial liquid and testing by interfering bacteria. Results DNA testing could be up to 10-3 min/mL, and 25 g samples could be tested for bacteria with a sensitivity of 100 CFU/25 g. Addition of 10 times of interfering bacteria would not reduce the detection sensitivity. Using the established method to screen the food purchased in the market and compare it with the standard method, the sensitivity of the established method was better than that of national standard method. Conclusion This method is accurate, sensitive and suitable for the detection of Pseudotuberculosis Yersinia in food.
标题：PCR-核酸试纸条法检测食品中假结核耶尔森菌
英文标题：Detection of Yersinia pseudotuberculosis in food by PCR-nucleic acid strips method
