婴幼儿配方奶粉中酪蛋白磷酸肽2种检测方法的比较
目的 比较婴幼儿配方奶粉中酪蛋白磷酸肽(casein phosphopeptides, CPPs)的2种主要检测方法。 方法 分别采用高效液相色谱法(high performance liquid chromatography, HPLC)和高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry, HPLC-MS/MS)检测婴幼儿配方奶粉中酪蛋白磷酸肽的含量。结果 HPLC操作简便, 检出限为7.5 mg/100 g, 日内精密度≤2.7%, 满足婴幼儿配方奶粉中酪蛋白磷酸肽的检测要求; 而HPLC-MS/MS检出限为1 mg/100 g, 日内精密度≤8.8%, 较HPLC具有更好的灵敏度。结论 HPLC高效准确, 实验操作简单, 适合于CPPs含量较高样品的检测分析; HPLC-MS/MS灵敏度高、特异性强, 适用于CPPs含量较低样品的检测。
Objective To compare 2 main methods for detecting casein phosphopeptides (CPPs) in infant formula. Methods High performance liquid chromatography (HPLC) and high performance liquid chromatography- tandem mass spectrometry (HPLC-MS/MS) were used to detect casein phosphopeptides in infant formula. Results High performance liquid chromatography was easy to operate, the detection limit was 7.5 mg/100 g, and the intra-day precision was less than 2.7%, which met the requirements for the detection of casein phosphopeptides in infant formula. The detection limit of high performance liquid chromatography tandem-mass spectrometry was 1 mg/100 g, and the intra-day precision was less than 8.8%, which had better sensitivity than high performance liquid chromatography. Conclusion HPLC method is effective and accurate, which is suitable for determination of the higher-content sample of CPPs. HPLC-MS/MS method has high sensitivity and specificity, which is suitable for the determination of the lower-content samples of CPPs.
标题：婴幼儿配方奶粉中酪蛋白磷酸肽2种检测方法的比较
英文标题：Comparison of 2 methods for detecting casein phosphopeptides in infant formula
作者：
胡蓓 浙江工业大学化工学院
赖世云 杭州帕匹德科技有限公司
张京顺 浙江省疾病预防控制中心
蒋易蓉 浙江大学生物系统工程与食品科学学院
任一平 浙江清华长三角研究院
中文关键词:酪蛋白磷酸肽,高效液相色谱法,高效液相色谱-串联质谱法,配方奶粉,
英文关键词:casein phosphopeptides,high performance liquid chromatography,high performance liquid chromatography-tandem mass spectrometry,infant formula,
发表日期：2018-07-19
- 文件大小:
- 41.36 MB
- 下载次数:
- 60
-
高速下载
|
|
