黄曲霉毒素B1时间分辨荧光定量检测体系适用性评价
目的 评价黄曲霉毒素B1时间分辨荧光定量检测体系(包括黄曲霉毒素B1时间分辨荧光免疫层析检测卡和荧光定量检测仪)的适用性。方法 用时间分辨荧光定量检测体系测定20个阴性样本中黄曲霉毒素B1的含量, 确定该检测体系的检出限(limit of detection, LOD)和定量限(limit of quantification, LOQ); 用2台荧光检测仪对6组含不同浓度黄曲霉毒素B1的大米和玉米阳性样本进行检测, 确定方法的准确性和台间差; 重复6次检测含中等浓度黄曲霉毒素B1的阳性样本和连续12 h检测国标检测限浓度的阳性标准溶液, 确定方法的重复性和稳定性。结果 该检测体系的LOD和LOQ分别为0.7和2.1 μg/kg; 该检测体系的检测值与大型仪器定值结果无显著性差异, 2台荧光检测仪的测值之间无显著性差异; 中浓度阳性样本和连续12 h测定检测限浓度的标准溶液的标准偏差未超过国家标准规定要求, 检测结果的稳定性和重复性满足国家标准要求。 结论 该时间分辩荧光定量检测体系能够准确检测玉米和大米样本中黄曲霉毒素B1含量。
Objective To evaluate the applicability of time-resolved fluoro-immunoassay quantitative detection system for aflatoxins B1 (including time-resolved fluorescence quantitative test strips and fluorescence quantitative detector). Methods The content of aflatoxin B1 in 20 negative samples were detected by time-resolved fluoro-immunoassay quantitative detection system, and the limit of detection (LOD) and limit of quantitation (LOQ) of the detection system were determinated. Six sets of rice and corn positive samples containing different concentrations of aflatoxin B1 were tested with 2 fluorescence detectors to determine the accuracy of the method and the difference between the stations. A positive sample containing a medium concentration of aflatoxin B1 and a positive standard solution for detecting the concentration of the national standard detection limit were repeated 6 times to determine the repeatability and stability of the method. Results The LOD and LOQ were 0.7 and 2.1 μg/kg, respectively. There was no significant difference between the measured value of the test system and the value of the large-scale instrument, and there was no significant difference between the measured values of the 2 fluorescence detectors. Conclusion The time-resolved fluoro-immunoassay detection system can accurately detect the content of aflatoxins B1 in rice and corn samples.
标题：黄曲霉毒素B1时间分辨荧光定量检测体系适用性评价
英文标题：Applicability evaluation of time-resolved fluoro-immunoassay quantitative detection system for aflatoxins B1
作者：
王文珺 北京智云达科技股份有限公司
刘磊 北京智云达科技股份有限公司
叶金 国家粮食局科学研究院
王松雪 国家粮食局科学研究院
谢刚 国家粮食局科学研究院
郑学玲 河南工业大学粮油食品学院
薛威 北京智云达科技股份有限公司
王覃 北京智云达科技股份有限公司
桑华春 北京智云达科技股份有限公司
中文关键词:黄曲霉毒素B1,时间分辨免疫荧光免疫层析,定量检测,
英文关键词:aflatoxins B1,time-resolved fluoro-immunoassay,quantitative detection,
发表日期：2018-06-27
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