辣椒粉中非法添加苏丹红色素快速检验分析
目的 建立辣椒粉中苏丹红色素快速检验分析方法。方法 以苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ为对照, 以正己烷-乙酸乙酯(7:1, V:V)为展开剂, 采用薄层色谱法进行定性鉴别; 以苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ为对照, Agilent C18(250 mm×4.6 mm, 5 μm)为色谱柱, 乙睛-0.2磷酸(92:8, V:V)为流动相, 流速为1.0 mL/min, 检测波长为495 nm, 柱温30 ℃, 采用高效液相色谱法进行检测。结果 建立薄层色谱法能很好分离各成分; 苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ的线性范围分别在16.49~245.59 μg (r=0.9999)、16.30~334.88 μg(r=0.9997)、16.81~478.02 μg(r=0.9999)、16.82~438.21 μg(r=0.9996); 平均回收率(n=6)分别为97.68%、98.35%、98.32%、98.14%。结论 本法简便可行、重复性好, 可用于辣椒粉中非法添加苏丹红色素的质量控制和快速检验。
Objective To establish a method for rapid detection and analysis of Sudan red in Capsicum annuum L. Methods Sudan red Ⅰ, Sudan red Ⅱ, Sudan red Ⅲ and Sudan red Ⅳ were used as control, hexane-ethyl acetate (7:1, V:V) was used as developing agent, and thin layer chromatography (TLC) was used for qualitative identification. Sudan red Ⅰ, Sudan red Ⅱ, Sudan red Ⅲ and Sudan red Ⅳ were used for control, the chromatographic column was Agilent C18 (250 mm×4.6 mm, 5 m), mobile phase was acetonitrile-0.2 phosphate (92:8, V:V), flow rate was 1.0 mL/min, detection wavelength was 495 nm, and column temperature was 30 ℃. Samples were detected by high performance liquid chromatography (HPLC). Results The established TLC method had a good separation effect on each component. The linear ranges of Sudan red Ⅰ, Sudan red Ⅱ, Sudan red Ⅲ and Sudan red Ⅳ were 16.49~245.59 μg (r=0.9999), 16.30~334.88 μg (r=0.9997), 16.81~478.02 μg (r=0.9999) and 16.82~438.21 μg (r=0.9996), respectively. The average recovery rates (n=6) were 97.68%, 98.35%, 98.32% and 98.14%, respectively. Conclusion This method is simple, feasible and reproducible, which can be used for quality control and rapid test of Sudan red pigments added illegally in Capsicum annuum L.
标题：辣椒粉中非法添加苏丹红色素快速检验分析
英文标题：Rapid test and analysis of Sudan red illegally added into Capsicum annuum L.
作者：
冯华 遵义市食品药品检验所食品生产检验科
王样培 贵阳中医学院药学院
王世俊 遵义市食品药品检验所食品生产检验科
郑凰雅 遵义市食品药品检验所食品生产检验科
中文关键词:辣椒,苏丹红,非法添加,快速检验分析,
英文关键词:Capsicum annuum L,Sudan red,illegally added,rapid test and analysis,
发表日期：2018-03-29
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