肽核酸-荧光原位杂交快速鉴定化妆品中的铜绿假单胞菌
目的 建立一种化妆品中铜绿假单胞菌(Pseudomonas aeruginosa)的快速检测方法。方法 基于铜绿假单胞菌的16S rRNA序列设计铜绿假单胞菌特异性肽核酸(peptide nucleic acid, PNA)探针PA-16S-1, 并对其探针进行灵敏度和特异性验证, 建立并优化固相肽核酸荧光原位杂交(peptide nucleic acid-fluorescence in situ hybridization, PNA-FISH)检测方法。结果 采用PNA-FISH法对300份化妆品样本进行检测, 检测到2株铜绿假单胞菌和1株金黄色葡萄球菌阳性样品, 与传统生化方法结果完全一致。结论 PNA-FISH方法是一种快速、敏感、特异的致病菌检测技术, 对控制和提高化妆品的卫生质量具有重要意义。
Objective To establish a method for rapid detection of Pseudomonas aeruginosa in cosmetics. Methods The Pseudomonas aeruginosa species-specific peptide nucleic acid (PNA) probe PA-16S-1 was designed based on the conserved regions in 16S rRNA sequences, and its specificity and sensitivity were experimentally verified. The peptide nucleic acid fluorescence in-situ hybridization (PNA-FISH) method was developed for specific detection of Pseudomonas aeruginosa. Results Three hundred cosmetic samples were detected by PNA-FISH, 2 strains of Pseudomonas aeruginosa and 1 strain of Staphylococcus aureus positive samples were identified, which were exactly the same as the results of traditional biochemical identification. Conclusion The established PNA-FISH identification method provides a fast, sensitive, and specific detection of pathogenic bacteria in cosmetics, which has an important significance to improve the sanitary quality of cosmetics.
标题：肽核酸-荧光原位杂交快速鉴定化妆品中的铜绿假单胞菌
英文标题：Rapid identification of Pseudomonas aeruginosa by peptide nucleic acid fluorescence in-situ hybridization
作者：
李可 浙江出入境检验检疫局
张晓峰 浙江出入境检验检疫局
吴姗 浙江出入境检验检疫局
方莹 浙江出入境检验检疫局
帅江冰 浙江出入境检验检疫局
杨兰花 浙江出入境检验检疫局
中文关键词:肽核酸-荧光原位杂交,鉴定,铜绿假单胞菌,化妆品,
英文关键词:peptide nucleic acid-fluorescence in situ hybridization,identification,Pseudomonas aeruginosa,cosmetics,
发表日期：2016-11-22
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