小米中姜黄染色的快速检测
目的 建立一种快速检查小米中姜黄染色的检测方法。方法 样品经乙酸-甲醇溶液超声提取后过滤, 滤液蒸干, 经硼酸-草酸溶液显色后检查姜黄染色的存在, 通过超高效液相色谱法-二极管阵列检测器(ultra performance liquid chromatography-diode array detector, UPLC-DAD)对样品的姜黄染色进行定性、定量分析。结果 显色反应具有较高的灵敏性。姜黄素在1.0~20 μg/mL浓度范围(样品含量4~80 mg/kg)时线性关系良好, 方法精密度RSD值小于2%, 方法平均回收率为99.8%。结论 该方法快速、准确、灵敏, 能够适合用于小米中姜黄染色的定性、定量检查。
Objective To establish a method for detection of turmeric coloration in millet. Method Samples were extracted with acetic acid-methanol solution, then filtered, and then the obtained solution was evaporated and colored with boric acid-oxalic acid solution to prove the existence of turmeric. The filtered solution was detected by ultra performance liquid chromatography coupled with diode array detector (UPLC-DAD) for qualitative and quantitative analysis. Results The methodology experiments revealed that the coloration displayed sensitively. Turmeric had a good linear relationship in the concentration range of 1.0~20 μg/mL(equivalent to amount of 4~80 mg/kg), the value of precision (RSD) was less than 2%, and the average recovery was 99.8%. Conclusion The established method is rapid, accurate and sensitive, which is suitable for qualitative and quantitative detection of turmeric coloration in millet.
标题：小米中姜黄染色的快速检测
英文标题：Rapid detection of turmeric coloration in millet
作者：
郭建博 陕西省食品药品检验所
朱小红 陕西省食品药品检验所
王松 陕西省食品药品检验所
宋莉 陕西省食品药品检验所
牟霄 陕西省食品药品检验所
吕卓 陕西省食品药品检验所
陈蓓 陕西省食品药品检验所
刘海静 陕西省食品药品检验所
中文关键词:小米,姜黄染色,超高效液相色谱法-二极管阵列检测器,
英文关键词:millet,turmeric coloration,ultra performance liquid chromatography-diode array detector,
发表日期：2017-04-12
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